When dna fragments are separated using gel electrophoresis and transferred to a blot it is called a:____.

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Answer 1

When DNA fragments are separated using gel electrophoresis and transferred to a blot it is called a southern blot.

In the field of molecular biology, we can define southern blot as a method through which a particular DNA fragment is detected from amongst a DNA sample.

The method of southern blotting has important uses such as the detection of diseases, for finding out a DNA segment in a DNA library.

In the method of southern blotting, the DNA sample is first cut by using restriction enzymes. Then the sample is run by the method of gel electrophoresis due to which the smaller segments move at a faster speed than a larger one along a matrix. The DNA segments are then transferred to a blot. The DNA is marked by a tag, referred to as a probe, using radioactive, fluorescent or any other technique. This tag will allow the detecting of our desired DNA segment when a complementary segment is run referred.

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Related Questions

fecal microbiota transplantation is superior to fidaxomicin for treatment of recurrent clostridium difficile infection

Answers

Fecal microbiota transplantation (FMT) is usually recommended for remedy of recurrent Clostridium difficile contamination (rCDI). We performed a single-center randomized trial to evaluate the effects of FMT with those of fidaxomicin and vancomycin.

All sixty four sufferers acquired their assigned remedy. The mixture of scientific resolution and negative consequences from the check for CD were observed in 17 sufferers given FMTv (seventy one%), 8 sufferers given fidaxomicin (33%), and three sufferers given vancomycin (19%; P = .009 for FMTv vs fidaxomicin; P = .001 for FMTv vs vancomycin; P = .31 for fidaxomicin vs vancomycin). clinical decision become found in 22 sufferers given FMTv (92%), 10 patients given fidaxomicin (42%), and 3 patients given vancomycin (19%; P = .0002; P < .0001; P = .thirteen). consequences did not fluctuate significantly between patients who received FMTv as their preliminary remedy and sufferers who acquired rescue FMTv. there was 1 extreme adverse occasion that might were associated with FMTv.

Disclaimer:- your question is incomplete, please see below for complete question.

fecal microbiota transplantation is superior to fidaxomicin for treatment of recurrent clostridium difficile infection. explain?

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In a similar experiment 40 years earlier, embryologist Hans Roux allowed the first cleavage to occur and then used a needle to kill just one blastomere. The embryo that developed from the remaining blastomere (plus remnants of the dead cell) was abnormal, resembling a halfembryo. How might the presence of molecules in the dead cell explain why Roux's result differed from the control result in Spemann's experiment?

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Embryonic environments include tidal pools, ponds, and wombs. As seen above, the embryo interacts with its environment and its developmental trajectory is guided by information from that environment. On a small scale, the environment of an embryonic cell consists of the surrounding tissue within the embryo, and the fate of that cell (e.g., whether it becomes part of the skin or the lens) is often determined by its Relies on immediate interaction with other components. ecosystem"".

His second research program in experimental embryology investigates how interactions between embryonic cells produce embryos. The development of specialized cell types is called differentiation. These apparent changes in cell biochemistry and function are preceded by processes involving the adherence of cells to specific fates. At this point, the cell or tissue is phenotypically indistinguishable from its unbound state, but its developmental fate is constrained. The commitment process can be divided into two phases (Harrison 1933; Slack 1991). The first stage is the unstable phase called specification. The fate of cells and tissues is determined when they can differentiate autonomously in a neutral environment such as a petri dish or test tube. (Environments are neutral with respect to development paths.) At this stage, bindings can still be unbound. The second level of commitment is determination. A cell or tissue is considered deterministic if it can differentiate autonomously when transferred to another region of the embryo.

Even in this situation, if we can differentiate according to our original destiny.

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The major biological source of oxygen, which many organisms require when they break down glucose, is actually a by-product of?

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The major biological source of oxygen, which many organisms require when they break down glucose, is actually a by-product of photosynthesis.

Glucose is a monosaccharide. It is also the main source of direct energy for many plants and animals. In humans, glucose can be found dissolved in the blood. The molecular formula of glucose is C₆H₁₂O₆.

Photosynthesis is the process of formation of one's own food using the inorganic raw materials like sunlight energy, water and carbon dioxide. The food formed is in the form of sugars and, water and oxygen are released as by-products. The photosynthesis occurs in plants, some bacteria and fungi.

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The diagram shows a cross section of a eukaryotic cell.

How do the nucleus and ribosomes work together?



a
The ribosomes perform chemical reactions that supply the nucleus with needed nutrients.
b
The ribosomes build proteins based on the genetic instructions they receive from the nucleus.
c
The nucleus creates proteins and lipids that the ribosomes then transport to other organelles.
d
The nucleus sorts and distributes the genetic information it receives from the ribosomes.

Answers

The nucleus and ribosomes work together because the ribosomes build proteins based on the genetic instructions they receive from the nucleus (Option b).

What are ribosomes?

Ribosomes are specialized organelles that generate proteins by using the instructions from messenger RNAs, which are templates of gene fragments found in the cell nucleus.

In conclusion, the nucleus and ribosomes work together because the ribosomes build proteins based on the genetic instructions they receive from the nucleus (Option b).

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Answer:

B

.) The ribosomes build protiens based on the genetic instructions they receive from the nucleus.

Explanation:

Patterns of dispersion can depend on scale. How might the penguin dispersion look from an airplane over the ocean?

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Patterns of dispersion can depend on scale. As you flew over densely populated islands and sparsely populated water, the penguin dispersal would most likely appear clumped.

What do dispersion patterns tell us about the population and its interactions?

Species dispersion patterns, also known as distribution patterns, this describes how individuals in a population are scattered in space and time. Individual organisms in a population can be more or less evenly spaced, randomly spread with no apparent pattern, or clustered in groups.

Patterns of dispersion and conclusions draw from these patterns :

Clumped, uniform, and random are the three patterns. A clumped pattern of animals could indicate mating activity or boost the efficiency of specific predators. A random pattern; an aggregated pattern, in which organisms congregate in clusters; or a uniform pattern, in which individuals are spaced about equally.

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Scientific study of the population cycles of the snowshoe hare and its predator, the lynx, has revealed that a. predation is the dominant factor affecting prey population cycling. b. hares and lyn x are so mutually dependent that each species cannot survive without the other. c. both hare and lynx populations are regulated mainly by abiotic factors. d. the hare population is r -selected and the lynx population is K -selected.

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According to the given statement:

Both hare and lynx population are regulated mainly by abiotic factors.

The correct option is c.

What exactly are abiotic factors?

Abiotic factors are non-living components of an ecosystem that influence their surroundings. Examples could be light, water, and temperature in a terrestrial habitat. Abiotic elements in a marine ecosystem would be salinity and ocean currents. A particular ecosystem is produced by the interaction of biotic and abiotic elements.

Why are abiotic elements important?

The non-living components of the environment, or abiotic influences, have a significant impact on living things. They can assist in figuring out topics like how tall trees grow, the locations of various animals and plants, and the causes of bird migration. Abiotic variables like water, sunlight, oxygen, soil, and temperature are crucial.

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quizlet cancer antigen-binding domains/sites of an antibody molecule are made up of heavy and light chains

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Answer:

ystsrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrr

Explanation:

rrrrysrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrrt

If the allele frequency for the recessive single allele that causes a particular rare hair color is 0. 02, how frequently would you expect the hair color to be present in humans?.

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If the allele frequency for the excessive single allele is 0.02, the particular rare hair color could be expected in 1 in every 50 individuals.

By dividing the total number of copies of all the alleles at that specific genetic locus in the population by the number of times the allele of interest is detected in a population, an allele frequency can be computed. If any allele frequency is 0.02 for a recessive allele, it would be expected to occur in about 1 individual out of every 50 individuals.

A fraction, a percentage, or a decimal can be used to express allele frequencies. The relative frequency of an allele at a certain locus in a population, represented as a fraction or percentage, is known as gene frequency or allele frequency.

In further detail, it is the percentage of chromosomes overall in the population that has that allele compared to the entire population or sample size. The most crucial genetic variation measurements are relative genotype frequency and relative allele frequency.

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using epstein-barr viral load assays to diagnose, monitor, and prevent posttransplant lymphoproliferative disorder

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In order to diagnose, track, and forecast posttransplant lymphoproliferative disease (PTLD) in immunocompromised graft recipients, Epstein-Barr virus (EBV) DNA quantification is being introduced into standard medical practice.

What is posttransplant lymphoproliferative disorder and what are its effects?Posttransplant lymphoproliferative disorder(PTLD) is an aggressive tumor that almost always contains EBV DNA in the neoplastic cells. It can be fatal if it is not identified and treated straight away.Implementing EBV load assays by real-time PCR is made easier by validated methods, available reagents, and automated tools. EBV DNA levels can be used to determine the clinical state of EBV-related neoplasia in either whole blood or plasma.Low virus loads are common in healthy transplant recipients, while high EBV loads are significantly linked to either existing or future PTLD. Simultaneous laboratory testing and histopathologic analysis of lesional tissue aid in the interpretation of slight increases in viral load. Circulating EBV levels are a useful, noninvasive technique for assessing the success of treatment since they reflect changes in tumor load in repeated samples. Serial testing enables early clinical intervention to stop the progression of frank PTLD in high-risk patients. A key method for treating PTLD is restoring T cell immunity against EBV, and innovative EBV-directed treatments are being investigated to prevent virus-driven neoplasia.

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The acid-fast stain is used to stain? bacteria with waxy cell walls. bacteria lacking cell walls. living bacteria. endospores. bacteria with capsules.

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The acid-fast stain is used to stain bacteria with waxy cell wall (option A).

What is acid-fast stain?

Acid fast stains are stains used to differentiate acid fast organisms such as mycobacteria.

The principle is based on the presence of mycolic acid in the cell wall of the so called acid fast bacteria as opposed to nonacid fast bacteria. A bacteria is said to be acid fast when it resists staining when stained with an acidic solution.

Acid fast bacteria will be red, while nonacid fast bacteria will stain blue/green.

Therefore, it can be said that acid-fast stain is used to stain bacteria with waxy cell wall.

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Which statement about human reproduction is false? a. Fertilization occurs in the oviduct. b. Spermatogenesis and oogenesis require different temperatures. c. An oocyte completes meiosis after a sperm penetrates it. d. The earliest stages of spermatogenesis occur closest to the lumen of the seminiferous tubules.

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The answer is the earliest stages of spermatogenesis occur closest to the lumen of the seminiferous tubules.

What is spermatogenesis?

The process by which sperm is produced in the testicles is known as spermatogenesis. The male gametes, or the sex cells that are responsible for fertilizing the ovum, are known as sperm.

Man's gametogenesis is spermatogenesis. The germ cells known as spermatogonia enable the creation of sperm in the gonads through the processes of meiosis and mitosis (the testicles). The epididymis is then where these gametes develop.

The epithelium of the seminiferous tubules, coiling ducts located inside the testicles, contains spermatogonia. Spermatogenesis, which in humans lasts between sixty-two and seventy-five days, is triggered by the gonadotropin-releasing hormone (GnRH).

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What are two advantages of a longer alimentary canal for processing plant material that is difficult to digest?

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The lengthened transit time through the canal permits more thorough processing, while the canal's expanded surface area offers more room for absorption.

What is the differences between carnivore and herbivore digestive systems?Carnivores and herbivores have radically distinct digestive processes. Typically, carnivores have a single stomach chamber and a straightforward digestive tract. A longer digestive system and many stomach chambers are common in herbivores. Ruminants are herbivores having several stomach chambers, including camels, deer, sheep, giraffes, and cattle. The teeth are where digestion starts. Large, flat teeth of herbivores are used to pulverize plant matter. Carnivores, on the other hand, mainly have pointed, sharp teeth that are useful for shredding flesh. After the plant material has been eaten, specialized bacteria in a herbivore's gut and the lengthier digestive system break it down.

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A STARCH MOLECULE IS LIKE A

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carbohydrate?

It is a polysaccharide, which means it is many individual sugars joined together.

The carotenoid pigments of some phototrophs interact with ________ to reduce its toxicity.

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The carotenoid pigments of some phototrophs interact with Singlet to reduce its toxicity.

Because carotenoids are fat-soluble substances, they are best absorbed when combined with fat. Cooking and chopping foods high in carotenes makes the nutrients more potent when they are absorbed into the bloodstream than some protein-rich foods and vegetables.

Xanthophylls and carotenes are the two main classes into which carotenoids are divided.

Carotenoids of both types have antioxidant effects. Some carotenoids can also be turned into vitamin A, which is necessary for human growth and health.

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Egg whites consist primarily of water and the protein albumin. When you fry an egg, why does the egg white turn from clear to white?.

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Egg albumen appears opaque and turns white when heated because the protein in it has a propensity to cogulate.

Long chains of amino acids that are raveled up into a carefully organized compact mass make up the proteins found in egg white. This mass is held in place and prevented from unraveling by chemical bonds and interactions between the amino acids in each protein. The process of denaturation, which occurs as an egg cooks, is caused by the heat breaking the bonds between the proteins.New interactions between these amino acids and the amino acids of nearby proteins emerge as these proteins chains unfold and entangle with other proteins, changing the texture and turning it white.

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how are rocks formed?

igneaus sedimentary metamorphic

Answers

Answer: Due to rise in temperature and pressure

Explanation: Rocks are formed due to rise in temperature and pressure. It involves larger rock masses. The main rock types are gneisses and schists which have a laminated banded appearance.

Stretch receptors called muscle spindles are located within which connective tissue layer?

Answers

Answer:

within extrafusal (skeletal) muscle fibers

Explanation:

Suppose the digger wasp had returned to her original nest site, despite the pinecones having been moved. What alternative hypotheses might you propose regarding how the wasp finds her nest and why the pinecones didn't misdirect the wasp?

Answers

Perhaps the wasp lacks visual signals. It's also possible that wasps distinguish natural to their habitat but not exotic things like pinecones.

How do wasps recognise landmarks?

Tinbergen researched the wasp Philanthus triangulum, popularly known as the beewolf. This is because wasps feast on bees. Wasps deposit their eggs in subterranean brooding chambers dug by females into suitable soft ground.

Tinbergen was previously aware that an emerging wasp performs an orienting flight in which she circles her nest many times before going out to forage.

Tinbergen placed the pinecones away from the nest and waited for the wasp to return to see if she was orienting to visual signals.

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What drives motility in amoebozoa?
a. flagella
b. pseudopodia
c. cilia
d. amoebozoa are non-motile

Answers

Pseudopodia drives motility in amoebozoa.

Amoebozoa are motile microorganisms that are either free-living or parasites that can cause diseases to human hosts.

Amoebozoa consist of pseudopodia that are responsible for the ability of the amoebozoa to move freely and are also involved in ingestion.

Pseudopodia reach out towards a substrate and attach to it from the anterior side and after this attachment, the pseudopodial membrane separates from the posterior side, and at the same time, the cytoplasm of the amoebozoa moves towards the new side resulting in the movement of amoebozoa from one place to another.

Amoebozoa commonly do not possess cilia and flagella and thus the absence of cilia and flagella means that they are not involved in the motility of amoebozoa.

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The discovery of ________ in plants has led to a deeper understanding of how cells protect themselves from stress responses and, of course, viral infections.

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The discovery of small RNA molecules that can silence genes in plants has led to a deeper understanding of how cells protect themselves from stress and viral infections. This knowledge is being used to develop new ways to protect crops from diseases and to improve our understanding of how plants resist stress.

RNA interference (RNAi) is a process in which cells use small RNA molecules to silence genes. This process is important for preventing the spread of viruses and protecting cells from stress.

In plants, RNAi plays an important role in resistance to viral infections. When a plant is infected with a virus, the plant produces smallRNA molecules that target the virus's genetic material. These smallRNA molecules silence the virus's genes, preventing the virus from replicating.

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Osmoreceptors that stimulate the release of antidiuretic hormone and initiate the thirst response are found in the?

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The thirst response are found in the hypothalamus of most homeothermic organisms that detects changes in osmotic pressure.

Most homeothermic creatures include osmoreceptors, which are sensory receptors that typically reside in the hypothalamus and are capable of detecting changes in osmotic pressure. Osmoreceptors can be found in a number of tissues, including two circumventricular organs: the subfornical organ and the vascular organ of the lamina terminalis.The anterior hypothalamus is home to the main osmoreceptors that regulate AVP secretion and thirst, and injuries to this area in animals lead to hyperosmolality through a combination of reduced thirst and osmotically enhanced AVP secretion.The anterior hypothalamus contains the majority of the central receptors, including the subfornical organ and the organum vasculosum laminae terminalis, two of the circumventricular organs.

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If science is based on facts, can we consider superstitions (traditional beliefs) as scientific? why or why not? give one example of a superstitious belief that you think is not scientific.

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Answer:

Superstitions are self-imposed selfish beliefs, something imaginary yet unimaginable disbelief, or mere beliefs. But science – the most well-known and popular word of this era. Science as is believed to be something realistic which even makes the unreal real; the unachievable achievable.

Explanation:

Predict what would happen if Ca²⁺ was injected into an unfertilized sea urchin egg.

Answers

The fertilization would be impacted if calcium was put into a sea urchin egg that had not yet been fertilized. Without the sperm entering the cell, the calcium in the egg causes the creation of a fertilization envelope. As a result, fertilization is stopped.

The marine invertebrate class Echinodermata and the kingdom Animalia both include sea urchins. They are fertilized by the merging of male and female gametes, which produces a zygote.

The fertilization envelope is a barrier to fertilization that stops an egg from being fertilized by more than one sperm cell. When cortical granules discharge their contents outside the egg, it is created.

When drugs are administered into the embryo of the frog before the neural tube is formed, it can block the functioning of the microfilaments. The contractions of the microtubules reduce significantly, and therefore, the folding of the neural plate does not occur, and the neural tube does not form.

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Cholesterol is the precursor molecule for all steroids in the body. Cholesterol is the precursor molecule for all steroids in the body.

a. True

b. False

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True, did this on a quizlet hope it helps

the basic unit of structure for all organisms is called the

Answers

Answer:

cells unit

Explanation:

The method that stimulates people to be creative by meeting with others and participating in an organized group experience is known as?

Answers

According to the research, the correct option is brainstorming. The method that stimulates people to be creative by meeting with others and participating in an organized group experience is known as brainstorming.

What is brainstorming?

It is an unstructured technique for work groups to inspire creative and effective problem solving by encouraging group members to brainstorm ideas or proposals to resolve an issue.

In this sense, this group work technique is aimed at finding solutions and developing new projects, exploiting the creativity of each person.

Therefore, we can conclude that according to the research, the correct option is brainstorming. The method that stimulates people to be creative by meeting with others and participating in an organized group experience is known as brainstorming.

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Rna contains equal proportions of g and c nucleotides. true false

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Erwin Chargaff  disproved an earlier hypothesis that the four bases were present in equal proportions, and dispelled the view that DNA was too simple and uniform to hold genetic information.

Does RNA contain C and G?

An RNA molecule has a backbone made of alternating phosphate groups and the sugar ribose, rather than the deoxyribose found in DNA. Attached to each sugar is one of four bases:

adenine (A)uracil (U)cytosine (C) or guanine (G).

With this information, we can conclude that Erwin Chargaff analyzed the base composition of DNA from diverse sources and discovered consistent ratios of the bases among all the sources.

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which of these is involved in the chemical digestion of protein? pancreatic amylase pancreatic protease salivary sucrase pancreatic nuclease

Answers

The substance that is involved in the chemical digestion of proteins is pancreatic protease (option B).

What is chemical digestion?

Digestion is generally the process, in the gastrointestinal tract, by which food is broken down into simpler substances that can be utilized by the body.

However, chemical digestion specifically involves breaking down food molecules via chemical means e.g. enzymes.

Enzymes are globular proteins that catalyse a biological chemical reaction. Protease is a specific kind of enzyme that cuts or cleaves proteins.

Therefore, the substance that is involved in the chemical digestion of proteins is pancreatic protease.

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How does the fertilization envelope form in sea urchins? What is its function?

Answers

Following the initial sperm-egg encounter, the paracrystalline protein fraction (PCF), which is produced from cortical granules, and the egg surface vitelline envelope (VE) combine to create the sea urchin fertilization envelope (FE).

Strongylocentrotus purpuratus FEs were used to extract a major protein fraction that contained seven major polypeptides, despite the fact that mature FEs are physicochemically hardened after insemination.

These major, separated components were immunologically cross-reactive with the major polypeptides in PCF and isolated cortical granules.

It is possible that only VE components are covalently connected based on the immunological crossreactivity of antibodies made against extracted, core FEs with isolated VEs but not with PCF.

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Why is the position of lung tissues within the body an advantage for terrestrial animals?

Answers

It has the benefit of preventing the moist respiratory epithelial surface from drying out in the air, or desiccation.

The main function of the respiratory system is to exchange carbon dioxide for oxygen in the body's tissues. The three main parts of the human respiratory system are the lungs, trachea, and nasal cavity. All aerobic species require oxygen for their metabolic activities to take place. The process by which organic molecules are broken down to produce ATP is known as cellular respiration. An adequate supply of oxygen is necessary for the aerobic respiratory machinery of Kreb's Cycle and the Electron Transport System to efficiently convert stored organic energy into energy locked in ATP. Carbon dioxide is produced during cellular digestion and needs to be removed from the cell. Carbon dioxide must leave the cell and oxygen must enter for there to be a gas exchange. Animals have organ systems that facilitate gas exchange and the movement of gases to and from exchange sites.

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A nearby skating park charges $4 for skates and an hourly rate for each hour.The sign on the right gives the prices for up to 3 hours of skating. Which linearequation represents the given information where C is the total cost and h is thenumber of hours spent in the park?1 hour2 hours3 hours$15$30$45 The most common technique used to measure oxygen consumption in an exercise physiology laboratory is? Which expression is equivalent to |8|+|-7|? true or false: if price and convenience of two products are equal, the product customers associate with the highest consumption utility is likely to have the highest demand. According to the law of ______________________________, the price of copper is directly related to the rate of its production. A culture that is very relaxed about time and does not consider punctuality to be important is considered ______. which element of malpractice occurs when the nurse does not act as a reasonable, prudent person would have acted in a similar circumstance? president hoover signing the let to the reduction in forign trade, hurting the american economy even more weegy What form of government that is widely used throughout Europe today, started in Ancient Greece?A:DemocracyB: TheocracyC: DictatorshipD:MonarchyPLEASE HELP FAST tom kemper is the controller of the wichita manufacturing facility of prudhom enterprises, inc. the annual cost control report is one of the many reports that must be filed with corporate head- quarters and is due at corporate headquarters shortly after the beginning of the new year. kemper does not like putting work off to the last minute, so just before christmas he prepared a preliminary draft of the cost control report. some adjustments would later be required for transactions that occur between christmas and new years day. a copy of the preliminary draft report, which kem- per completed on december 21, f points P, Q, R, and S are collinear. Point Q is between P and R, R is between Q and S, and . and PQ = RS . If PS = 33 and PR 25, what is the value of QR? rawlco began the month with $220,000 in total assets. what total assets would be reported on the balance sheet after events (a)(f)? 2 Write 386 in expanded form jessica is an economist. she loves being an economist so much that she would do it for a living even if she only earned $36000 per year. instead, she earns $103000 per year. how much producer surplus does jessica enjoy? Conflicts between people or groups of people aconflict binterpersonal conflicts cinternal conflicts dhazing Hyro Moore had a dream about what piece of equipment The new immigrants quickly assimilated into american culture, eager to learn the english language and expand their communities to include fellow immigrants from other cultures. True or false?. Please beo I will give brainliest to you consider the production possibilities frontier for an economy that produces only sofas and cars. as the economy moves from point a to point d, is the opportunity cost of cars increasing, constant, or decreasing? and it is equal to? Read the passage from "Your Everyday Zombie" by Christie Wilcox.The tale above is just one of many real-life horror stories experienced by animals everyday. In all of the cases I'm about to present, once the host is infected, they are theliving dead. They have no hope of recovery, no chance for redemption. They willperform their acts as needed by their parasitic puppet master, and only once theirbodies have served their function will the zombie be released into the sweet arms ofeternity.Why would the author include the phrase parasitic puppet master? Choose the best answer.O To emphasize that the host is now being controlled by something else.O To make the article seem scarier.O To emphasize that the parasite is a human.O To emphasize that the host is now completely in charge of the parasite.