What are proteins and how do you detect them ?

Answers

Answer 1

Answer:

Immunological-based methods such as quantitative enzyme-linked immunosorbent assays (ELISA), Western blotting and dot blotting are very common and sensitive assays for protein detection, and they use antibodies that react specifically with entire proteins or specific epitopes (e.g., fusion tags) after cell lysis.


Related Questions

Giving brainliest!!!!!!!

Answers

Examine and describe cells
examine and describe cells! :)

If human red blood cells are placed in a 0 osmoles/L solution, a 100 osmoles/L solution, a 300 osmoles/L solution, and a 500 osmoles/L solution, then _________________ will be the isotonic solution.

OPTIONS:
0 osmoles/L solution
100 osmole/L solution
300 osmoles/L solution
500 osmoles/L solution

Answers

If human red blood cells are placed in solutions of different osmolarity, then the 300 osmoles/L solution will be the isotonic solution.

An isotonic solution to the red blood cell would be a solution that has the same solute concentration as the red blood cell.

Isotonic solutions are different from hypotonic and hypertonic solutions. Hypotonic solutions would have lower solute concentrations than the red blood cell while hypertonic solutions would have higher concentrations than the red blood cells.

The red blood cells of humans have a certain amount of solute in their cell sap which has been approximate to be between 286 and 295 osmoles/L. Thus, the only solution close to being an isotonic solution to these cells would be the 300 osmoles/L solution.

More about osmolarity can be found here: https://brainly.com/question/8144230

Answer: 300 osmoles/ L solution

Explanation:

The 300 osmoles/ L solution is the solution closest to the osmolarity of a normal human red blood cell.

This means that the 300 osmoles/ L solution is the isotonic solution. If added to human red blood cells, the shape and size of the red blood cells with remain as they were, unchanged (unlike if a hypotonic/ hypertonic solution was added.)

What types of organisms do alcohol fermentation ? Summarize this process,

Answers

Answer:Two kinds of organisms can do alcohol fermentation: bacteria and yeast (yeast, by the way, are fungi). Humans “use” alcohol fermentation in another way, co-opting it to make bread, beer, and wine.

Explanation: i took the test

Which statement best explains why the atmosphere is considered an open system?
A.
There is no definite boundary separating it from outer space.
B.
Energy and matter can enter and leave.
C.
Its molecules can move freely, without resistance.
D.
It can accept an unlimited amount of energy.

Answers

Answer:

thw anser is A

Explanation:

Explain the term “soil profile” (needs to be a paragraph)

Answers

Answer:

A soil profile is a vertical section of soil

Explanation:

Is 2Na + H2O → 2NaOH + H2 a balanced equation?

Answers

Answer:

Yes

Explanation:

Its reaction type is single replacement.

Which of the following choices is a component in the community of a wetlands
ecosystem?

A. Water
B. Sunlight
C. Soil
D. Frog

Answers

Answer: Water

Explanation:

A community is the occupied space by a population of two or more species. Water is a community component of a wetland ecosystem. Thus, option A is correct.

What is a wetland ecosystem?

Wetland is a type of ecosystem that comprises water reservoirs and has an excess of stored water in the form of bogs, marshes, and swamps. A wetland ecosystem includes many biotic and abiotic factors that interact and live together.

The wetland ecosystem is characterized by the presence of water throughout all the years. It is a valuable ecosystem that absorbs excess precipitation so as to avoid flooding and soil erosions. Hydrology, soils, and biology are the main components of the ecosystem.

Therefore, option A. water is the component of the wetland ecosystem.

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the difference between Eukaryotic and prokaryotic cells​

Answers

Answer:

Eukaryotic cells are cells with a nucleus (such as animal and plant cells) whereas Prokaryotic cells are cells without a nucleus (such as a bacterium cell)

Explanation:

The motivation to plan and initiate the movement is located in area.

Answers

Motor cortex ! Have a great day

In the picture down below is my question

Answers

Answer:

The answer is Telophase

The answer is telophase

Why do metals dissolve in acids?

Answers

Answer:

In water or acids, the metals trade places with hydrogen. The hydrogen escapes as a gas, and metal atoms, no longer attached to the object from which they came, dissolve in solution.

sorry if I'm wrong but I wrote this in my hw and it was right so ye

electrons are negatively charged. acids are positively charged. when an acid in contact with a metal, the acid steals free electrons away. That loosens the bonds holding metal atoms in place, so the metal dissolves.

When cells divide they make two ____ cells.

A- daughter
B- son
C- dad
D- mom

Answers

Answer:

A.) daughter cells

Explanation:

Nice pfp. <3

Answer:

the correct answer would actually be mom or parent cell

Explanation:

mitosis is a process of nuclear division in eukaryotic cell that occurs when a parent cell divides to produce two identical daughter cells during cell division mitosis refers specifically to the separation of the duplicated genetic material carried in the nucleus

Which of these is the smallest?
cells
atoms
molecules
cell membranes

Answers

Answer:

The correct answer is atoms :)

Explanation:

atoms are most likely the correct answer considering that they are so small that they will never touch anything.

please help me out with this newsela question

Answers

Answer: the answer is a

Explanation:because it is stating that you have to see if there is anything involving chemical substances.

Select the correct answer.
The difference in concentration of materials across a space is called the
A active transport
B temperature
C concentration gradient
D equilibrium

Answers

The Correct choice is C

The difference in concentration of materials across a space is called the Concentration gradient .

Explain the importance of gene regulation in prokaryotic cells.

-Explain the importance of gene regulation in eukaryotic cells.

Answers

Answer:

Prokaryotic cells can only regulate gene expression by controlling the amount of transcription. As eukaryotic cells evolved, the complexity of the control of gene expression increased. For example, with the evolution of eukaryotic cells came compartmentalization of important cellular components and cellular processes.

Explanation:

Hope this helps.

Beaker A contains 6 liters of water and 1 osmole of glucose. Beaker B contains 10 liters of water and 2 osmoles of glucose. Which one has the greater osmolarity and by how much?

Answers

From the ratio of glucose osmole to the volume of water in the two beakers, beaker B would have the greater osmolarity.

The term osmolarity refers to the number of particles of solutes or osmoles of substances per liter of solution.  

In this case, the osmolarity of beaker A can be calculated as;

               1 osmole/6 liter

                                = 0.167 osmolar

The osmolarity of beak B can be calculated as:

                              2 osmoles/10 liter

                                        = 0.2 osmolar

Thus, beaker B with 0.2 osmolar has the greater osmolarity when compared to beaker A with 0.167 osmolar.

More on calculating concentrations can be found here:  rainly.com/question/17329736

Answer: B

beaker A is 0.16 Osm/L and Beaker B is 0.2 Osm/L Beaker B has a greater osmolarity by 0.04 Osm/L

Explanation:

What did Louis Pasteur's experiment contribute to cell theory?

Answers

Louis Pasteur contributed to cell theory by disproving the notion of spontaneous regeneration.

He was the first scientist to prove that cells can only form from pre-existing cells. He did this by creating an experiment that showed cells would only grow in broth if air was exposed.

Answer:

D) The proof that cells only come from other cells

Explanation:

I took the test and got 100%

HURRY PLS

A student observes a plant cell and an animal cell under a microscope. he records the following observations.

Answers

Answer:

I think it's A.

Explanation:

If you look at the chart, responses to water loss or gain doesn't change the plant cell at all. But if you look at the animal cell, if it gains or loses water, it shrinks or increases in size depending on what happened. Plus, I think it's really the only answer choice that makes sense given the whole table and then looking at the other answers that don't really fit in with the descriptions.

Answer: its a

Explanation:

How does anesthesia work?

Answers

anesthetics induce a different sort of anesthetic state, one of general insensibility to pain. The patient loses awareness yet his vital physiologic functions, such as breathing and maintenance of blood pressure, continue to function. Less is known about the mechanism of action of general anesthetics compared to locals, despite their use for more than 150 years. The most commonly used general anesthetic agents are administered by breathing and are thus termed inhalational or volatile anesthetics. They are structurally related to ether, the original anesthetic. Their primary site of action is in the central nervous system, where they inhibit nerve transmission by a mechanism distinct from that of local anesthetics. The general anesthetics cause a reduction in nerve transmission at synapses, the sites at which neurotransmitters are released and exert their initial action in the body.

match each muscle of the arm with the correct label

Answers

Answer: 1 - Brachialis 2 - Brachioradialis 3- Pronator teres 4 - Palmaris Longus 5 - Extensor carpi radialis 6 - Extensor digitorum

Explanation:

The correct match of the muscles has been stated below and these are as follows:

1 - Brachialis, 2 - Brachioradialis, 3- Pronator teres, 4 - Palmaris Longus, 5 - Extensor carpi radialis, 6 - Extensor digitorum.

What is muscle?

Muscle is defined as the group of the tissue and contraction has been defined as an action potential travels along the nerves to the muscle. The beginning of the muscle contraction which has been occurs with the signal generated from the nervous system.There are generally two types of the muscle contraction and the situation occur when the muscle contracts and the change in the size takes place.

Isotonic concentric contraction h been known as the type of muscle contraction in which muscle has been shortening takes place. Isotonic eccentric contraction has been the type of the muscle contraction in which the muscle lengthening whilst it is under tension.

Therefore, 1 - Brachialis, 2 - Brachioradialis, 3- Pronator teres, 4 - Palmaris Longus, 5 - Extensor carpi radialis, 6 - Extensor digitorum.

Learn more about muscle on:

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Developing and using models, making observations, and using mathematics apply to

a. science only.
b. engineering only.
c. both science and engineering.
d. neither science nor engineering.

Answers

I would have to go with C it makes more sense to me

Which of these processes is MOST directly involved in the resorption of a tadpole’s tail during development?
A. Apoptosis
B. Mitosis
C. Phagocytosis
D. Protein synthesis

Answers

Answer:

A. Apoptosis

Explanation:

Select all that is true about the ocean floor.
A)We know more about Mars and the Moon that we do about the ocean floor.
B)The ocean floor is relatively flat and featureless. C)The goal is to have the entire ocean floor mapped by 2030.
D)We use sonar and satellites to map the ocean floor.
E) Although there are mountain ranges underwater, they are not as tall as ones in the United States.​

Answers

Answer:

A,B and D

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please help me figure this out

Answers

K n n mkzlalzoxkxm I bbxkzlzkznzkzmzixn so the answer will be extended
The answer is flexed

Place the following in the correct order as they occur in CELL RESPIRATION.

1. In cytosol, 6-C glucose and 2 ATP split into two 3-C molecules (glycolysis)
2. Acetyl Co-A enters mitochondria and drops off 2-C molecules
3. 2-C molecule combines with 4-C molecule and breaks into two 3-C molecules
4. Resulting 3 C molecule is stabilized BEFORE entering the mitochondria
5. Krebs cycle spins twice; each time stripping 1 carbon to make Co2 as a by-product
6. With each cycle of Krebs, electrons and H+ ions pop off, are picked up by NADH and FADH2 and taken to ETC
7. Electrons and h+ ions pop off and are picked up by NADH
8. 4 molecules of ATP are generated
9. Coenzyme A attaches to 2-C molecule making Acetyl Co-A
10. 1 C is stripped from 3-C PGA and makes Co2

Please help ;(

Answers

Electrons and h+ ions pop off and are picked up by NADH. 1 C is stripped from 3-C PGA and makes Co2.Coenzyme A attaches to 2-C molecule making Acetyl Co-A.Resulting 3 C molecule is stabilized BEFORE entering the mitochondria.Acetyl Co-A enters mitochondria and drops off 2-C molecules.In cytosol, 6-C glucose and 2 ATP split into two 3-C molecules (glycolysis).2-C molecule combines with 4-C molecule and breaks into two 3-C moleculesKrebs cycle spins twice; each time stripping 1 carbon to make Co2 as a by-product.With each cycle of Krebs, electrons and H+ ions pop off, are picked up by NADH and FADH2 and taken to ETC4 molecules of ATP are generated.

a hydrogen atom shares electrons with an oxygen atom in water molecule forming a strong​

Answers

Answer: Covalent Bond

Explanation: A covalent bond occurs when electrons are shared between two atoms, in this case the hydrogen atom is sharing an electron with the oxygen atom, creating a strong covalent bond.

I hope this helps!

Which process creates food for plants in order to maintain homeostasis? Breathing O Cell division Cellular respiration Photosynthesis​

Answers

Answer:

Photosynthesis

Explanation:

Photosynthesis

How do enzymes affect the reactions in living cells?

Answers

Answer:

enzymes speed up the reaction

Explanation:

Enzymes increase the rate of biochemical reactions by reducing the amount of activation energy needed for reactants to start reacting.

Answer:

Activation energy decreases.

Explanation:

The way enzymes work is by lowering the activation energy required for reactions to take place within living cells.

Note on chemical communication in insects in general

Answers

Answer:

Chemical communication plays an important part in the lives of insects, and particularly in lives of those that live in groups or social organizations. Aggregation pheromones are designed to bring individuals together into groups which may be temporary in sub-social insects, or permanent in social insects.

Explanation:

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