If your nanodrop is giving you a plasmid DNA concentration of 178.9 ng/µL what is the volume of sample, you need to take in order to have 10 ng DNA final concentration?


Please help!!
Thanks.

Answers

Answer 1

The volume of the solution is obtained as 0.056 µL.

What is the concentration?

We know that the term concentration refers to the amount of substance that is present in a sample. In this case, we are told that you have a plasmid DNA concentration of 178.9 ng/µL. We are now being asked for the volume of the sample that is needed so that we can have 10 ng DNA final concentration.

Let us now look at the parameters that we have from the question and these are;

Concentration =  178.9 ng/µL

Mass of the sample = 10 ng

Volume of the sample = ???

We know that the volume is going to be obtained as the ratio of the mass to the concentration of the solution from the data that we have and have been itemized in detail above.

Given that we have;

Volume = mass/concentration

Volume = 10 ng /178.9 ng/µL

Volume = 0.056 µL

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explain why something is biodegradable

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If products do not decompose, ii will commonly dwell in landfills and so much area will be taken up. Biodegradable means that there is the abililty to be decomposed and there is no harm in the ecosystem produced by this process. Materials or objects can be broken down to smaller parts and absorbed into the soil that will serve as nutrients.

Define gluconeogenesis and lipogenesis. Under what conditions do they predominantly occur?

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The process of producing glucose (sugar) from lipid (fat) or protein breakdown products, as well as from its breakdown products is called gluconeogenesis whereas the creation of fatty acids from nonlipid precursors is known as lipogenesis.

Gluconeogenesis predominantly occurs both in the kidneys and the liver, because phosphoenolpyruvate carboxykinase is inhibited by ADP. As a result, gluconeogenesis is encouraged in cells that are abundant in ATP and biosynthetic precursors.

The liver, adipose tissue, small intestine, kidneys, and mammary glands are the primary sites of lipogenesis.  Additionally, it happens in blood lipoproteins and the digestive system. It is a metabolic pathway that is activated by the high availability of carbohydrates and is used to break down extra carbohydrates.

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True or FalseDarwin theorized that the forces seen today in the biological world such as reproduction, inheritance, and competition gradually produce the whole diversity of life on Earth.

Answers

The correct answer is FALSE. Although Darwin, indeed recognized reproduction and competition as important drivers of global biodiversity, he did not included the role of inheritance. Rather, he emphasized the role of competition and natural selection.

Which of the following processes is not associated with sexual reproduction?OOOOmeiosisfertilizationbinary fissionrecombination

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Of the following processes the one that is not associated with sexual reproduction is the binary fission, that is a asexual reproduction by a separation of an organism of the body into two new bodies.

What type of muscle typically forms a lining for various organs?A) Rough muscleB) Smooth muscleC) Cardiac muscleD) Skeletal muscle

Answers

The type of muscle that typically forms a lining for various organs is the smooth muscle. (option B)

The process of transporting materials in a cell involves a positive change in the amount of free energy in the cell. Which of the following best describes how this process affects the cell?The process is non-spontaneous and causes an increase in entropy.The process is non-spontaneous and moves the cell away from equilibrium.The process is spontaneous and can be used to do work in the cell.The process is spontaneous and releases heat to the cell's surroundings.

Answers

The process is non-spontaneous and causes an increase in entropy.

The process is not automatic even thoug it involves transportation of materials in a cell. There is a change in the amount of free energy but there is entropy.

1. Why is the band in lane 2 not as bright as lane 3? They are both restriction digests on two different DNA plasmids.

2. Lanes 5-6 are PCR products. How do the inserts reflect on the restriction digest in lanes 8-9?

3. Are PCR products smaller or larger than corresponding restriction digest fragments?

Answers

Larger DNA molecules travel more slowly across the gel matrix during electrophoresis than smaller ones do. As a result, the DNA molecules divide into different bands according to their respective sizes. The quantity of DNA that builds up in a band determines how black the band is.

Which PCR products travel more quickly, big DNA fragments or little ones? Why?

Because DNA is negatively charged, it gravitates toward the positive electrode. All DNA fragments flow through the gel at the same speed because they all have the same mass-to-charge ratio.

A PCR result must frequently be further altered by cleavage with restriction enzymes. Without any DNA purification, restriction enzyme digestion may be carried out quickly and easily right in the PCR mixture.

The PCR primers' structure determines the size of the PCR result. To amplify an area of interest from a double-stranded DNA template, the user designs PCR primers. Always two primers are present, and they are made to border the area.

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Did the WES2 station move at a constant speed since 1995?

Answers

Answer:

probably not

Explanation:

When a velocity's magnitude and direction do not alter over time, it is said to be constant.

What is meant by Constant speed?

Constant speed refers to a speed that remains constant throughout the duration of the motion. Constant speed is demonstrated in our example of using cruise control while driving a car.

When a velocity's magnitude and direction do not alter over time, it is said to be constant. In other words, this occurs when an object's rate of change in location remains constant throughout time.

An object is considered to be moving at a constant speed when it covers the same distance in the same amount of time. When moving at a constant place, an object covers a certain distance in a fixed amount of time. S = dt is a formula that can be used to express the speed.

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Which has the highest potential energy - glucose or glycogen?

Answers

Glucose is referred to as the compound which has the highest potential energy.

What is Potential energy?

This is referred to as the type of energy which is possessed by a body by virtue of its position and is depicted by the state of rest of a body or compound.

Glucose and glycogen are compounds which helps supply energy to the cells of the body so as to enable them perform their daily activities. Glucose however has the highest potential energy because it has more bonds and also means there is more energy available to perform different types of reactions.

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Indicate the location and function of the plasma membrane, as well as an analogy of it.

Answers

Plasma membrane it's located in the exterior of all cells.

The plasma membrane has several functions: first, it's the barrier between the interior of the cell and the rest of the environment; secondly, it protects the cell and gives it support; it also regulates what substances pass to the interior of the cell.

If the cell was a ballon, the plasma membrane would be the latex and all the air inside would be the cytoplasm.

The anatomy of spiders and insects is structurally identical.

Please select the best answer from the choices provided:

True
False

Answers

Answer:
False
Explanation:
Spiders are divided into two segments, the abdomen and the cephalothorax. However, insects are divided into three or more. Take note of an ant the next time you see one, it will be divided into three different segments.

True or FalseNatural selection and mutation have proven to have been able to produce a new kind of organism.

Answers

The statement is false.

New kinds of organisms are impossible to make because we don’t have the necessary information, (programming) or material to be able to construct kinds of organisms.

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Answers

Photosynthesis and cellular respiration are, in a way, opposite reactions.

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Answers

When we consider species like the ones the question tells us about, we usually one faces an example of predation, since squirrel can behave as tertiary consumers for spiders, which are then secondary consumers. So the roles they develop in the environment can be named as population maintenance (especially for the squirrels, because they can control the wolf spider population, and spiders can allow them to continue their population growth).

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Answers

The correct answer is D. biological agents. Mistakes when DNA is replicated is a common biological source of mutations.

The complimentary base strands for TGA CAA CGA GGT ACT are start

Answers

The complimentary base strands for TGA CAA CGA GGT ACT are start

ACT GTT GCT CCA TGA.

Complimentary base strands- Adenine and guanine, as well as thymine and cytosine, are the complimentary bases in DNA. A nitrogen-containing base, such as guanine, adenine, thymine, or cytosine, a monosaccharide sugar termed deoxyribose, and a phosphate group make up each nucleotide in DNA.

The complimentary bases may unite to form a bond. This aids in holding the DNA molecule's two antiparallel strands together just to form the helix. The complimentary base pairing rule refers to this.

According to Chargaff's criteria, DNA from all cells of all creatures should contain a 1:1 ratio of pyrimidine & purine bases. More specifically, the amounts of guanine and adenine should be equivalent to cytosine and thymine, respectively.

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What is the role of environmental factors, such as altitude and heat, in very long-distance run or other performances lasting one to four hours? becomes limiting in terms of energy production?

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The role of  environmental factors in very long-distance run or other performances is that the longer the performance, the greater the chance that environmental factors play a role in the result and is a limiting factor.

What is Energy?

This is referred to as the ability or the capacity to do work and examples include the following: potential, chemical energy etc.

The longer the performance, the greater the chance that environmental factors play a role in the outcome and they become limiting in terms of energy production because they are able to restrict or influence certain activities needed for its generation.

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What is the difference between a prophage and a provirus?

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A prophage is a viral genome that infects bacterial cells, thus it integrates into a bacterial genome; while a provirus is a viral genome that integrates into an ekcaryotic genome.

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Answers

The primary structure occurs when the protein structure is only a chain of amino acids, with no folds or twists. Therefore, b) is incorrect.

This primary structure then twists folds in itself, creating the structure that's folded and held in shape by hydrogen bonds. That's the secondary structure, and whats is folded in it is the amino acids. Therefore, c) is the correct answer.

The tertiary structure is a three-dimensional structure, shaped by interactions between the R groups of the amino a cids. To form this structure, the entire secondary structure elements (not the amino acids) are folded together. Therefore, a) is also incorrect.

The quaternary structure is a closely pack arrangement of several protein chains, each with its own primary, secondary and tertiary structure. Therefore, d) is wrong as well.

Dimples are dominant; no dimples are recessive. Mr. Dowling is heterozygous for dimples, and Mrs. Dowling is also heterozygous for dimples. What are the possibilities of their offspring?

Answers

The possibilities of the offspring to have dimples is 75% (TT, Tt and Tt) and no dimples are 25% (tt) because both the parents are heterozygous dominant.

What are the three laws of mendelian genetics ?

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Law of Dominance is one of Mendel's three proposed laws of inheritance, which also includes Law of Inheritance. The law that enforced segregation. The Principle of Independent Assortment is a Law.

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A man (A+) and a women (B+) are expecting a baby together. Make a two-trait (dihybrid) Punnett square and determine the probability of each blood type in their offspring. A+ ___:16B+ ___:16AB+ ___:16O+ ___:16A- ___:16B- ___:16AB- ___:16O- ___:16

Answers

A+ _3__:16

B+ __3_:16

AB+ _3__:16

O+ _3__:16

A- _1__:16

B- __1_:16

AB- _1__:16

O- __1_:16

Tabulate in detail the comparison among Simple Diffusion, Facilitated Diffusion, and Active Transport across a cell membrane.

Answers

The cell has several types of membrane transport alternatives, being the simple diffusion, facilitated diffusion, and active transport one of the options for the cell to exchange molecules between internal and external environment.

The types of cell transport:

1 - Simple Diffusion: is a process that does not need energy (passive), used to transport small and nonpolar molecules from a region of higher concentration into a region of lower concentration (in the gradient concentration direction);

2 - Facilitated Diffusion: is a process that does not need energy (passive), used to transport larger ions and polar molecules with the help of carrier proteins or pore proteins (transport proteins);

3 - Active Transport: There is two types of active transport, primary active transport is used for molecules moving against their gradient coupled to the hydrolysis of ATP, and secondary active transport is for molecules going with more molecules against their gradient. It is a process that needs energy (active) to happen, going always against the gradient of concentration of the cell.

In order to maintain a high concentration of
potassium inside a cell (with a low
concentration outside) which of the following
must occur?
O passive transport
O active transport
O diffusion
O osmosis

Answers

Answer:

Passive transport does not require the cell to expend energy and involves a substance diffusing its concentration gradient across a membrane.

Sorry for the bad English, love from Vanuatu!

Explanation:

Taste buds are located within the circumvallate and fungiform papillae. A) TrueB) False

Answers

There are four types of tongue papillae: (1) filiform, which is thin and wired shape and no taste buds, (2) fungiform, which is mushroom-shaped near the middle and with taste buds, (3) foliate, which is leaf-shaped near the sides and with taste buds, and (4) circumvallate papillae, which is whorls at the sides and with taste buds.

ANSWER: TRUE. There are taste buds in the fungiform and circumvallate papillae.

I need help with this practiceThe subject is about the invasive species KudzuTake your time to review the photo and then answer in your own words shortly

Answers

• They can be introduced to an area by ship ballast water, accidental release, and most often, by people. Invasive species can lead to the extinction of native plants and animals, destroy biodiversity, and permanently alter habitats.

,

• Biodiversity plays a crucial role in human nutrition through its influence on world food production, as it ensures the sustainable productivity of soils and provides the genetic resources for all crops, livestock, and marine species harvested for food.

I extracted DNA from strawberry. I had to answer: - Why did the DNA separate to the top after adding the isopropyl alcohol? - Does all of your food have DNA? Explain your reasoning. - Why is it important for scientists to be able to extract DNA? Provide two examples.

Answers

1. Isopropanol is a compound used to dissolve lipids and as a dehydrating agent. When mixed with DNA, they compete for water causing the DNA to dehydrate and separating the mixture into two phases.

2. Every living organism contains DNA. This includes plants and animals, and therefore most of the food we eat.

3. DNA contains the hereditary information of all living organisms.

For example, in humans it is important to extract DNA for the identification of diseases, their causes and possible treatments.

In food, DNA extraction can help in the production of transgenic foods.

Look up one prion disease and describe it here. What population does this disease infect? How is it usually transmitted? How common is it? What are the major symptoms? Be sure to cite your sources.

Answers

Kuru is a Transmissible spongiform encephalopathy which was formerly common among the Fore people of Papua New Guinea. This disease is caused by a prion, i.e., a protein that can trigger other proteins in the brain to fold abnormally. The abnormally folded proteins in the brain cause neurodegeneration which ultimately lead to death. The main symptom of the Kuru disease is the loss of coordination and control over the muscle movements. The transmission of Kuru was because of a ritual in which Fore people consumed body parts of their family members just after their death. The Fore people stopped to eat human meat in the early 1960s, causing the kuru epidemic to decline dramatically. It is not certainly known if the last kuru victim died in 2005 or 2009.

Which allele will be expressed when both a dominant and recessive allele arepresent in an organism?A. the two alleles will mixB. dominant onlyC. recessive only

Answers

The answer is the letter B. dominant only

*Dominant allele is expressed exclusively in heterozygous organisms (presence of dominant and recessive allele).

*Recessive traits are expressed when the organism has a homozygous recessive allele.

Food Web AssignmentDirections illustrate the flow of energy within the aquatic system below by completing each direction below.SquidBlue WhaleSealKrillPenguinZooplankton1.) Draw in 8 arrows to show how the energy moves to create a food web. You may have to do a little research to help youmake all the correct connections!2.) Where would phytoplankton and the Sun belong in this food web? Draw it as well as an arrow connecting each one accurately.3.) How would bacteria nit into this food web? Describe how you would draw it in. 4.) Classify each of the 7 organisms in the aquatic food web, as well as bacteria, as a producer, consumer or decomposer by writing each into the correct category below. • Producer - • Consumer - • Decomposer -

Answers

For the first and second question we have:

Squid eats: Krill

Blue whale eats: Krill

Seal eats: Krill, Squid, Penguins (Only leopard seals)

Krill eats: Zooplankton (and phytoplankton)

Penguin eats: Krill, squids

Zooplankton eats: no one (in the picture, but they eat phytoplankton)

For the third question: Bacteria enter as decomposers in the food web.

Finally, for the fourth question we have:

Producers: Phytoplankton

Consumers: squids, blue whale, seal, krill, penguin and zooplankton

Decomposer: Bacteria

Pleaseeee answer!!
Four friends wrote down their descriptions of sleet and hail.

Dan: Sleet is small, frozen ice pellets that melt and refreeze as they pass through different layers of air, while hail is usually formed as larger chunks of ice during thunderstorms.
Julie: Sleet is a mixture of solid ice pellets and liquid rain, while hail is usually formed as water droplets at above freezing temperatures.
Patsy: Sleet is liquid water in the form of drops that fall through air temperatures above freezing, while hail is a mixture of rain and snow that usually forms during thunderstorms.
Timothy: Sleet is formed when water vapor freezes to form ice crystals that join to form flakes, while hail is formed as a chunk of ice during thunderstorms.

Which friend wrote the most complete and accurate description of sleet and hail?

a) Dan
b) Julie
c) Patsy
d) Timothy

Answers

The answr is b the reason I already took this
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