Compound light microscopes and transmission/transmitted light microscopes produce an image based on light transmission through the sample. Explain how the fluorescence microscope layout differs from that of transmission microscopes. Include the source of the light visualized in fluorescence microscopy in your answer.

Answers

Answer 1

The fluorescence microscope differs from the compound light, and transmitted light, not so much because of the structure, but instead because of how light is used to generate an image/ see the sample.

In the fluorescence microscope, the light does not go through the sample per se, instead, a filter is needed, it works with a higher wavelength, in this case, the light rays go to the sample and excite the species marked with the fluorescence substance, that is to say, the sample must be previously treated so it can absorb light and re-emitted as fluorescence (electromagnetic radiation) therefore creating an image, of light. This is possible due to the proprieties of some molecules, such as fluorochromes.


Related Questions

Earth's___ and _____ affect the relative amounts of solar radiation regions receive

Answers

Answer:

Earth's distance and tilt affect the relative amounts of solar radiation regions receive.

Explanation:

Earth's distance and tilt affect the relative amounts of solar radiation regions receive. There are no options provided in the question, but this is the correct answer. The earth's orbit is not circular and the earth rotates in a tilted orbit. Whenever the south pole is tilted towards the sun, then the south pole will get more solar radiation than the north pole. The Earth absorbs most of the energy reaching its surface and a small fraction is reflected. In total, approximately 70% of the incoming radiation is absorbed by the atmosphere and the Earth's surface while around 30% is reflected back to space and does not heat the surface.

a mosquito repellent is sprayed on one arm and the other arm is not sprayed. The repellent is an example of in independent or a controlled variable.

Answers

A mosquito repellent which is sprayed on one arm and the other arm is not sprayed, is an example of independent variable.

A mosquito repellant is a chemical substance applied on the skin, clothes or any non-living object to avoid the appearance of mosquitoes on that surface. It is made of constituents like DEET, metofluthrin, oil of lemon-eucalyptus, picaridin, etc.

Independent variable in an experiment is that parameter which is changed. It has the potential to alter the results of the experiment as the effect on the experiment are influenced by this variable. It is called independent because no other variable can make changes in its effect.

To know more about independent variable, here

brainly.com/question/1479694

#SPJ1

What is the function of the ozone layer?A. To absorb carbon dioxide.B. To trap infrared radiation.C. To block UV radiation.D. To recycle sulfur dioxide.

Answers

The correct answer is C. To block UV radiation. Ozone filters the majority of UV spectra, so it protects organisms from its effects.

Which of the following pairs of base sequences could form a short stretch of a normal double helix of DNA?
A) 5'-purine-pyrimidine-purine-pyrimidine-3' with 3'-purine-pyrimidine-purine-pyrimidine-5'
B) 5'-AGCT-3' with 5'-TCGA-3'
C) 5'-GCGC-3' with 5'-TATA-3'
D) 5'-ATGC-3' with 5'-GCAT-3'
E) All of these pairs are correct.

Answers

The pairs of base sequences could form a short stretch of a normal double helix of DNA is:

D) 5'-ATGC-3' with 5'-GCAT-3'

In DNA, the code letters are A, T, G, and C, which stand for the chemical substances adenine, thymine, guanine, and cytosine, respectively. Under normal occasions, the nitrogen-containing bases adenine (A) and thymine (T) pair collectively, and cytosine (C) and guanine (G) pair collectively. The binding of those base pairs forms the structure of DNA.

Because of the base pairing, the DNA strands are complementary to each different, run in opposite guidelines, and are referred to as antiparallel strands.

Complementary base pairing is important in DNA because it lets the base pairs be arranged in the maximum energetically favorable way; it's miles crucial in forming the helical structure of DNA. it's also critical in replication as it allows semiconservative replication.

You could determine the sequence of a complementary strand if you are given the series of the template strand. These strands are complementary, with each base in one sticking to its partner on the alternative. The A-T pairs are linked by means of two hydrogen bonds, whilst the G-C pairs are connected through three hydrogen bonds.

Learn more about Complementary base pairing here: https://brainly.com/question/19670398

#SPJ4

Evaluate the rate of cell division over time.

(This is for a project about cell division using gizmo for different diagrams and this is the final question needing to be answered in 3-5 sentences. all I have so far is that the rate goes up over time but dont know what else to put)

Answers

The rate of cell division over time is calculated as an average for the entire meristem by dividing it with the total cell flux. Total cell flux may be characterized as the number of cells in the meristem.

What is Cell division?

Cell division may be defined as a type of biological process through which a parental cell divides into two or more daughter cells. There are two types of cell division are there:

MitosisMeiosis.

According to the context of this question, the availability of the nutrient plays an active role in the process of cell division. It can increase the population of cells by doubling the rate of promoting growth and division of respective cells.

Therefore, the rate of cell division over time is calculated as an average for the entire meristem by dividing it with the total cell flux.

To learn more about Cell division, refer to the link:

https://brainly.com/question/796780

#SPJ1

Help assp…..
What are the divisions of the PNS? (Choose all that apply)

A.autonomic nervous system
B.reflex arc
C.parasympathetic nervous system
D.somatic nervous system

Answers

it is B C and D

Hope this helps

Reflex arc, parasympathetic nervous system and somatic nervous system are the divisions of peripheral nervous system.

What are the functions of peripheral nervous system?

The peripheral nervous system is one of two components that make up the nervous system of bilateral animals, with the other part being the central nervous system. The PNS consists of nerves and ganglia, which lie outside the brain and the spinal cord.

The peripheral nervous system refers to parts of the nervous system outside the brain and spinal cord. It includes the cranial nerves, spinal nerves and their roots and branches, peripheral nerves, and neuromuscular junctions.

The peripheral nervous system is divided into two main parts: Autonomic nervous system (ANS): Controls involuntary bodily functions and regulates glands.

Learn more about peripheral nervous system:

https://brainly.com/question/23605940

#SPJ2

Oxygen and carbon dioxide molecules pass freely through cell membranes. Which factor determines thedirection most molecules will pass through the cell membrane?A the strength of the covalent bonds holding the gas atoms in the molecules.B the concentration of the gas molecules on each side of the membrane.the ionic charge on the outer shell of the gas molecules.D the size of the gas molecules.

Answers

There are three factors that determine whether a molecule can pass through a cell membrane: concentration, charge and size. The greater the difference in concentration, the more rapid the diffusion. Large and heavier molecules more more slowly so they diffuse more slowly.

B. the concentration of the gas molecules on each side of the membrane.

When an animal is hungry it hunts for food. In this case, what is the stimulus?A. hungerB. hunting

Answers

And stimulus is a change in the organism or the environment that triggers a reaction, in this example, hunger causes the animal to hunt, so hunger would be the stimulus.

A serum amylase is diluted 1/100 with a result of 45.0 U/L. What is the patient's actual amylase result?

Answers

A serum amylase is diluted 1/100 with a result of 45.0 U/L. The patient's actual amylase result is 4500 U/L.

Serum- Serum generally refers to the transparent fraction of any biological fluid, whether it comes from an animal or even a plant. For instance, the fluid released by serous glands is known as mammal serous (or serosal) fluid. The serum in blood is a clear, pale yellow liquid that is separated from of the blood and thus is free of blood cells & clotting components.

Amylase- The primary job of amylases is to hydrolyze all glycosidic linkages in molecules of starch, turning them to simple sugars. The three primary kinds of amylase enzymes—alpha, beta, and gamma—each affect the carbohydrate molecule in a different way.

To know more about the Amylase, click on the below link,

https://brainly.com/question/13488041

#SPJ1

Help asap…

1. Where is the thalamus located?

A.The cerebellum
B.The cerebrum
C.The brain stem
D.The diencephalon

Answers

D. the diencephalon is where the thalamus is located

D.

The thalamus is a paired gray matter structure of the diencephalon located near the center of the brain. It is above the midbrain or mesencephalon, allowing for nerve fiber connections to the cerebral cortex in all directions — each thalamus connects to the other via the interthalamic adhesion.

The law of _____ suggests that the orbit of planets is not circular but _____. Fill in the blanks: circles triangles ellipses rectangle

Answers

Answer:

Explanation:

Kepler's First Law: The orbit of a planet around the Sun (or a satellite around a planet) is not a perfect circle. It is an ellipse—a “flattened” circle. The Sun (or the center of the planet) occupies one focus of the ellipse. A focus is one of the two internal points that help determine the shape of an ellipse. The distance from one focus to any point on the ellipse and then back to the second focus is always the same.

The law of ellipses suggests that the orbit of planets is not circular but ellipses

Therefore your answer would be; Ellipses

Answer:

The law of ellipses suggests that the orbit of planets is not circular but elliptical.

Explanation:

Kepler's first law (the law of ellipses) explains that the orbit of the planets around the sun is not in a circular but elliptical path.

What is the name of the southern part of South America?

Answers

Answer:

Patagonia is the southern psrt of south america.

Tierra del Fuego would be the answer

ou are testing a patient's fecal sample for the presence of Clostridium difficile. Which of these staining procedures would give you the best evidence that the patient's sample contains bacteria from the Clostridium genus? Group of answer choicesendospore staingram stainsimple stainacid fast stain

Answers

As a protocol, the sample must first undergo primary biochemical tests, one of which is gram staining. And although this stain can help us to identify bacteria of the Clostridium genus, such as gram + sporulated bacilli, a more specific test would be the endospore stain, since the clostridium genus produces endospores that are also easy to isolate, since they are more resistant to temperature than the vegetative form (not sporulated) of the bacterium. An example would be the Schaeffer-Fulton stain.

how could the differences in these pictures be related to evolutionary fitness?

Answers

To be considered as fit for evolution processes, a species must be able to adapt in its environment and with capacity to reproduce sucessfully. Ambrosia, for example, is an invasive species. It has the abillty to promote accelerated evolution due to its adaptibility to its environment. Zea mays is an excellent species for genetic analysis. Quercus also has evolutionary potential because of its physiological characteristics.

What surface can only vacuum metal deposition be used on?

Porous surfaces

Non-porous surfaces

Metals

Fabrics

Answers

The surface that only vacuum metal deposition can  be used on is option  B: Non-porous surfaces.

What are Nonporous materials?

Nonporous materials are substances that do not permit the passage of liquid or air. Nonporous materials are those that are not porous, such as glass, plastic, metal, and varnished wood.

Note that  Vacuum lifting nonporous materials is much simpler than vacuum lifting porous surfaces, as the definition suggests. A proven method for creating latent fingerprints on non-porous surfaces is vacuum metal deposition (VMD).

Therefore, A variety of displays, such as flexible plastic packaging, plastic bottles, glass, and others, respond best to vacuum metal deposition (VMD).

Learn more about metal deposition from

https://brainly.com/question/1128134
#SPJ1

I need help with this practice problem solving My questions for you are at the top

Answers

Hi. We just had a session with this same question is there any doubt left?

How far apart has Africa and North America move apart since 2001

Answers

Africa and North America have moved apart since 2001 by a total of about 21 inches.

What is a Continent?

This comprises of about seven landmasses on the earth which is usually very large and discrete and comprises of different countries. Examples include Africa, Asia, North America, South America etc.

Continents in the world such as Africa and North America move apart since 2001 at a rate of 1 inch per year. Since we were told that it occurred in 2001 then the total years in which it occurred is 21 then =

1 inch × 21 = 21 inches.

Read more about Continent here https://brainly.com/question/22687489

#SPJ1

Which gas is transported by the circulatory system in humans and is USED BY cells during respiration to release energy stored in food? *A Carbon DioxideB NitrogenC HydrogenD Oxygen

Answers

In order to answer this question, we must remember a bit of cellular metabolism, when mitochondria carry out the process of cellular respiration we can see that in the electron transport chain the final acceptor of electrons is oxygen. Therefore the correct answer for the question is option D oxygen.

Explain at the chemical level why plants die when they are not watered.
Your answer must explain the importance of water to photosynthesis and
describe specifically how photosynthesis would be affected and
effectively shut down with the loss of water.

Answers

Answer:

All living things need to water to survive. This is because all living things are mostly made of water and since they lose water in different ways, they need to get more water to stay the same size. So if a plant does not get enough water, it will shrink. If it goes long enough without water it will die because the plant uses water for a lot of different jobs needed to keep the plant alive.

Draw comic strip should include an example of transcription  (DNA to mRNA) and an example of translation (mRNA to amino acid, and include tRNA) as well as the location of both in the cell.

Answers

We have to consider that the DNA is first transcribed into pre mRNA in the nucleus of the cell by the RNA polymerase. After this process, the non-coding fragments (introns) of the molecule are spliced by ribonucleoproteins (snRNP). Eventually, each end of the pre mRNA is modified, being a modified nucleotide named CAP joined to the 5' end, and a polyA (poly Adenine) tail at the 3' end before the now matured mRNA gets out of the nucleus to the cytoplasm.

At this point, the mRNA will be recognized by ribosomes, which will synthesize each group of three nucleotides (codons) into amino acids in a process called translation, by the action of tRNA, and finally joined into a new protein.

Note that A, P, and E are sites of the ribosomes, being A the position where first arrives the mRNA.

Fungi exist as single cells or as branching networks of multicellular filamentswith a very large surface area. What is the function of the filaments?A. ReproductionB. Nutrient absorptionC. Energy productionD. Photosynthesis

Answers

Micelium, which is formed by a number of filaments, has the main function of penetrating the substrate in search of food and nutrient. Moreover, it can suffer some modifications to form reproductive structures.

The correct option is: B. Nutrient absorption.

What is the difference between chromosomes, sister chromatids, non-sister chromatids, separated chromatids?

Answers

Chromosomes are structures of nucleic acids and protein carrying genetic information in the form of genes.

The chromosome is composed of two sister-chromatids.

During meiosis and mitosis, the sister chromatids are separated to separate half of the geentic material and create a new cell.

The difference between sister chromatids and non-sister chromatids is that sister chromatids have the same alleles in the same place and non-sister chromatids have different alleles in the same place. Non-sister chromatids will have the same length and configuration, but those different alleles will represent different types of the same characteristic (for example: different colors of fur). But they look the same as sister chromatids.

4. All enzymes have an optimal temperature at which they work as well as an optimal pH for their activity. Does this mean that enzymes only work at those specific temperatures and pH optimums? Briefly explain your answer using evidence from the unit that supports your answer.

Answers

Enzymes perform their functions within a specific temperature and pH range (which is specific for each enzyme in particular). In turn, within this range, there is a specific value at which they act in the most efficient way possible, which does not mean that they cannot on other values in the range. However, outside the limits of the range, the internal characteristic of enzymes can be affected and cause their denaturation, thus losing their capacity of action.


• How do you think the concept of the speed of light helps astronomers study the universe?Is the
speed of light in a vacuum the same regardless of where an observer stands? Explain your answer.

PLEASE HELP ME!!!!!

Answers

The cosmos is expanding at the quickest rate that astronomers have discovered. In actuality, nothing is known to move faster than the speed of light, which acts as a cosmic speed limit.

Regardless of the mobility of the source or the viewer, the  in a vacuum remains constant for all observers.

What does the idea of light speed mean?

speed of light, the rate at which light waves move through various substances. Specifically, the speed of light in a vacuum is presently calculated to be 299,792,458 meters per second. One of nature's basic constants is the speed of light.

To learn more about speed of light refer to:

https://brainly.com/question/100983

#SPJ13

3. What did you leam about the effects of salinity on of CO₂ on pH and how that Impa levels?​

Answers

Answer: When CO2 is absorbed by seawater, a series of chemical reactions occur resulting in the increased concentration of hydrogen ions. This process ...

Explanation:

If a city
were a model for a cell,
would wastewater be more
likely part of endocytosis or
exocytosis? Explain.

Answers

If a city were a model for a cell, would wastewater be more likely part of endocytosis.

Briefing:

The process of engulfing an object or particle with the cell membrane and bringing it inside the cell is known as endocytosis. The membrane completely encloses the substance by folding it over.

What is an endocytosis ?

Endocytosis is the process by which cells take in foreign material by encasing it in their membrane. The two primary kinds of endocytosis are ocytosis and receptor-mediated endocytosis.

What is endocytosis transport?

Endocytosis is a term that refers to a variety of active transport processes that carry particles into cells by encasing it in vesicles made of plasma membranes (endo = internal, cytosis = transport mechanism).

To know more about Endocytosis visit:

https://brainly.com/question/26500494

#SPJ13

A biologist found an asymmetrical organism. A part of the organism was injured, but it was able to repair itself through regeneration.

Because the organism is asymmetrical and it was able to regenerate, it’s probably
.....
, and belongs to the phylum
....
.

Answers

Answer: 1. The right answer is amphibian.

2.  The right answer is Mollusks.

Explanation:

1. Regenerating a limb is not possible in mammals, as in most vertebrates. But in amphibians such as the salamander yes!

This amphibian does it very well, capable of making tail, eye or snout too. For a very long time, scientists have been trying to understand this secret of the salamander, or that of the zebrafish, because the answer is scientifically interesting but also, of course, because we can hope to take advantage of it to imagine one day of medical treatment for injured members. Just over a year ago, an Australian team showed the role of macrophages, cells of the immune system.

2. Mollusks (Mollusca) are a branch of Lophotrochozoans animals. They are unsegmented animals, with bilateral symmetry sometimes altered. Their body usually consists of a head, a visceral mass, and a foot. The visceral mass is covered in whole or in part by a mantle, which secretes a calcareous shell. The nervous system includes a double periesophageal collar. The general cavity is more or less reduced to pericardium and nephridia.

Properties of Matter Unit Test
7 of 117 of 11 Items
Question
Which of the following statements is true?(1 point)
Responses

The density of water is 1 gram per milliliter, so objects with a relative density of 1 milliliter per gram will float.
The density of water is 1 gram per milliliter, so objects with a relative density of 1 milliliter per gram will float.

The density of water is 1 gram per milliliter, so objects with a relative density greater than 1 gram per milliliter will float.
The density of water is 1 gram per milliliter, so objects with a relative density greater than 1 gram per milliliter will float.

The density of water is 1 gram per milliliter, so objects with a relative density greater than 1 gram per milliliter will sink.
The density of water is 1 gram per milliliter, so objects with a relative density greater than 1 gram per milliliter will sink.

The density of water is 1 gram per milliliter, so objects with a relative density less than 1 gram per liter will sink.

Answers

The statement that is true is as follows: the density of water is 1 gram per milliliter, so objects with a relative density greater than 1 gram per milliliter will sink (option C).

What is density?

Density is the measure of the mass of matter contained by a unit volume. The density of a liquid substance is a determinant of whether the substance will float or sink in water.

Water is a liquid substance with a density of 1 gram per millilitres (1 g/mL). This suggests that any substance with greater density than that of water will sink while any substance with lesser density than water will float.

Density is a measure of how heavy something is compared to its size, hence, if an object is more dense than water it will sink when placed in water, and if it is less dense than water it will float.

Therefore, according to this question, objects with a relative density greater than 1 gram per milliliter will sink.

Learn more about density at: https://brainly.com/question/15164682

#SPJ1

True or false? Having an autoimmune disease will eventually lead to death.Select one:TrueFalse

Answers

Answer: False

Explanation: Having an autoimmune disease will not necessarily lead to death, many of them have a treatment which allows the patient to maintain his/her quality of life.

What’s the correct answer answer asap for brainlist

Answers

I think answer is option d
Answer: b. A reaction between multiple chemicals

Explanation:
A chemical bond is a connection between two surfaces or objects that have been joined together
Other Questions
wingate company, a wholesale distributor of electronic equipment, has been experiencing losses for some time, as shown by its most recent monthly contribution format income statement: sales $ 1,550,000 variable expenses 618,000 contribution margin 932,000 fixed expenses 1,025,000 net operating income (loss) $ (93,000) in an effort to resolve the problem, the company would like to prepare an income statement segmented by division. accordingly, the accounting department has developed the following information: division east central west sales $ 400,000 $ 610,000 $ 540,000 variable expenses as a percentage of sales 49% 32% 42% traceable fixed expenses $ 275,000 $ 327,000 $ 194,000 required: 1. prepare a contribution format income statement segmented by divisions. 2-a. the marketing department has proposed increasing the west division's monthly advertising by $21,000 based on the belief that it would increase that division's sales by 18%. assuming these estimates are accurate, how much would the company's net operating income increase (decrease) if the proposal is implemented? 2-b. would you recommend the increased advertising? Find the mean for the data set. 6, 14, 7, 4, 12, 8, 13, 4, 18, 14 What is the solution to the inequality?17 26OD. x< 26SUBMIT The radius of a circle is 8 inches. What is the area?Give the exact answer in simplest form. _____ square inches. (pi, fraction) Matthew filled two 20 oz. water bottles before he left home. At the end of the day, he has less than 8 oz. left. Write an inequality to determine how much water, z, Matthew drank. Which equation is true when x=10 but not when x= 10? Select all that apply. if (11,13) is an ordered pair of the function F(x), which of the following is an ordered pair of the inverse of F(x) which statement regarding the human genome is false? long stretches of repetitive dna are prominent at centromeres and ends of chromosomes. the human genome is smaller than that of the bacterium haemophilus influenzae. current estimates are that there are about 21,000 genes in the human genome. the human genome contains approximately 19% introns. Assume your salary is $24,000 per year and $50 for each computer you sell. What function represents your total pay for one year? Be sure to indicate any domain restrictions. Find a if (10-a )2 +(2a2)+(4a+7)=48 which of the following is a component of the monetary base? a) bonds held by banks, loans, and bank reserves b) currency in circulation plus bank reserves c) currency in circulation plus checkable deposits d) bonds held by banks plus checkable deposits e) the sum of currency in circulation, bank reserves, and checkable deposits A hotel is only as good as its management team. Discuss the pros and cons of having a small management team consisting of just three people. Would you like to work in a hotel with such a small management team? How many and what type of solution(s) does the equation have?6p2 = 8p + 32 rational solutions1 rational solutionNo real solutions2 irrational solutions Given the formula for the nth term, state the first 5 terms of each sequence.t1= 800, tn= -0.25tn-1 PLEASE SHOW UR WORK!!1. Jose made some pancakes. For every 6 cups of flour, he added 7 cups of milk. The ratio of flour to milk in Jose's pancakes is ____. (5 points) A. 6B. 7C. 6 over 7D. 7 over 62. For every 40 phone calls that Jess made in a month, she received 70 phone calls. What is the ratio in simplest form of the number of phone calls made to the number of phone calls received by Jess that month? (5 points)A. 3:4B. 4:3C. 7:4D. 4:73. Melissa needs to mix 2 cups of baking soda with 3 cups of vinegar to make a cleaning solution. How many cups of baking soda are needed for each cup of vinegar? (5 points)A. 2 over 3 cupB. 3 over 2 cupsC. 2 over 5 cupD. 3 over 7 cup4. The following table shows the bonus earned by Abel for selling different numbers of toys: Abel's Bonus Number of Toys Sold Bonus Earned 8 28 10 35 12 42 14 49 16 ? The missing number in the table is _____.5. The table shows the width and length of different pictures that Coby has enlarged for the school yearbook:Width (inch) Length (inch)3 76 149 21Based on the table, what is the length of a picture that has a width of 18 inches? A. 36 inchesB. 42 inchesC. 54 inchesD. 90 inches A cat is stuck in the tree and the fire department needs a ladder to rescue the cat. The fire truck available has a 95-foot ladder, which starts 8 feet above ground. Unfortunately, the fire truck must park 75 feet away from the tree. If the cat is 60 feet up the tree, does the cat get rescued? If not, what ladder length is need to allow the cat to be rescued? A candy box is made from a piece of cardboard that measures by inches. Squares of equal size will be cut out of each corner. The sides will then be folded up to form a rectangular box. What size square should be cut from each corner to obtain maximum volume?. Need help with a math word problem for homework. Thank you in advance A gene or trait that appears or expresses itself over a recessive trait is called the anstwer find the value of XA. 11 41inB. 11 inC. 33 inD. 35 in