A student who was trying to determine the concentration of escherichia coli in a sample completed a total plate count assay. After plating 1ml of the 10-6dilution tube and incubating the plate at 37oc for 24 hours the student counted 210 colonies on the plate. What is the cfu/ml of the original sample?.

Answers

Answer 1

Colony forming units/ml (CFU/ml) of the original sample are 2.1 * 10^8 CFU/mL according to the total plate count test.

The number of colonies created per given mL of the final diluted sample of the cultivated bacteria is measured by the colony forming units/ml (CFU/ml) of a given sample of cultured bacteria.

CFU = number of colony-forming units on the countable plate*1/FDF

where

FDF = final dilution factor

The sum of all dilution factors from the initial sample to the countable plate results in the final dilution factor.

As per the given data:

FDF = 1*10^-6

Number of the colony-forming units on the countable plate = 210

The colony forming units/ml (CFU/ml) of the original sample = 210*1/1*10^-6

The colony forming unit/ml(CFU/ml) of the original sample is 2.1*10^8 CFU/ml

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Related Questions

Calculate the frequency of a wave with a wavelength is 6.0 x 10​-7​ m.

Answers

The frequency of a wave with a wavelength of 6.0 x 10-⁷m is 5.0 × 10¹⁴Hz.

How to calculate frequency?

The frequency of a wave can be calculated by dividing the wavelength by its wavelength as follows:

f = v/λ

Where;

f = frequencyv = speed of light (3 × 10⁸m/s)λ = wavelength

According to this question, a wave has a wavelength of 6.0 x 10-⁷m. The frequency can be calculated as follows:

f = 3 × 10⁸ ÷ 6.0 × 10-⁷

f = 5.0 × 10¹⁴Hz

Therefore, the frequency of a wave with a wavelength of 6.0 x 10-⁷m is 5.0 × 10¹⁴Hz.

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An object with a mass of 15.3kg accelerates 3.5.0 m/s2

Answers

The force that acts on the body is 53.55 N

What is the force?

According to the Newton law, the force is the product of the mass and the acceleration of a body. This is in accordance the Newton second law of motion. We now have to obtain the force that acts on the body.

Mass of the object =  15.3kg

Acceleration of the object = 3.5.0 m/s2

Force = 15.3kg * 3.50 m/s2

Force = 53.55 N

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Convert 150 mg to grams

Answers

Answer: 0.15 grams

Explanation:

Answer: 0.15 grams

Explanation:

to convert milligrams to grams the mass value is divided by 1000, because there are 1000 mg in one g.

which of the following statements are true about the properties of both molecular and ionic compounds? select all that apply.

Answers

They are neutrally charged

They typically do not conduct electricity in solid state

What are ionic compounds?

A chemical compound known as an ionic compound in chemistry is one that contains ions bound together by the electrostatic forces known as ionic bonding. Despite having both positively and negatively charged ions, or cations and anions, the molecule is generally neutral.

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Which bonding type has circular symmetry with respect to the bond axis?
a) sigma bond
b) pi bond
c) delta bond
d) covalent bond
e) ionic bond

Answers

Sigma Bond has circular symmetry with respect to the bond axis

Sigma bonds are formed by  head-on overlapping between atomic orbitals. These are the strongest type of covalent chemical bond. Pi bond are formed by the lateral overlap of two atomic orbitals. Delta bond is formed by the  overlap of four lobes of one involved atomic orbital with four lobes of the other involved atomic orbital.

Covalent Bond is formed by equal sharing of electrons between two atoms. Ionic Bond is formed by the transfer of one or more electrons between atoms.

Sigma bonds are symmetric with respect to the inter-nuclear axis. They also have  circular symmetry with respect to the bond axis.

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Which is the most accurate statement of the phenomenon underlying 'hydrophobicity', the clustering of oily molecules in the presence of water?

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The most accurate statement is: water molecules lose opportunities to form bonds with each other when 'interrupted' by oily/hydrophobic ones.

Hydrophobic molecules or hydrophobes have very low solubility in water.

They are nonpolar molecules and do not interact with water molecules.

Oily molecules clump up together in a water because like that, they have minimal interaction with water.

Hydrogen bonds between water molecules (H₂O) will be broken so that the hydrophobes can interact.

When bonds are broken, heat in the system is greater (an endothermic reaction).

Hydrophobic interactions between hydrophobic molecules are spontaneous.

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Which is an example of potential energy?

A
Water behind a dam

B
A dart stuck in a dartboard

C
Plants using photosynthesis

D
A cow chewing grass

Answers

The example of Potential energy is the water behind a dam.

Due to its higher elevation than the water on the other side of a hydroelectric dam , the water behind one stores gravitational potential energy. This potential energy is transformed into kinetic energy when the water falls, turning turbines to produce electricity.

The water held behind the dam has Potential Energy because of its position water in the reservoir is on height and when something is on a certain height the Potential Energy P.E. increases.

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using this indicator, we will attempt to determine the concentration of various acids depending on the amount of protons available for donation. using a single acceptor base (naoh), we will titrate the acid to the point where it turns pink, representing an equal mixture of acid and base.

Answers

Using phenolphthalein indicator, we will attempt to determine the concentration of various acids depending on the amount of protons available for donation. using a single acceptor base (naoh), we will titrate the acid to the point where it turns pink, representing an equal mixture of acid and base

An Phenolphthalein indicator is a ‘dye’ that shows a change in colour when in contact with acids and bases. This tells us whether the substance we are testing is an acid or base with respect to the change of colour. Phenolphthalein indicator when comes in contact with acid and base they give intense pink colour.  

Phenolphthalein Colour in acid (pH  < 7) give Colourless and Colour at pH = 7 also give Colourless, Colour in base(pH > 7) gives Pink colour that's why using Phenolphthalein indicator we will attempt to determine the concentration of various acids depending on the amount of protons available for donation. using a single acceptor base (naoh), we will titrate the acid to the point where it turns pink, representing an equal mixture of acid and base.

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What is the end result of a dichotomous key?

Answers

Answer:

A dichotomous key provides users with a series of statements with two choices that will eventually lead to the correct identification of the organism

Explanation:

this should help

Complete the flowchart about the scientific method.

Answers

1.observation

2.hypothesis

3.Experiment

4 solution

5 theory

The independent variable

The dependent variable

write the symbol for every chemical element that has atomic number greater than 70 and atomic mass less than 179.5 u

Answers

The elements and there symbols are as follows: Oxygen ⇒ O fluorine ⇒ F Neon ⇒ Ne Sodium ⇒ Na.

What is Oxygen ?

When a hydrogen atom loses or receives an electron, a hydrogen ion is produced. A positively charged hydrogen ion (or proton) can combine with other particles easily, therefore it is only observed alone when it is in a gaseous condition or a place that is almost particle-free.

The bare hydrogen ion cannot live freely in solution due to its extremely high charge density, which is approximately 21010 times that of a sodium ion. Instead, it rapidly hydrates, or binds, with other molecules. The IUPAC recommends using the phrase "hydrogen ion" to refer to all hydrogen ions and its isotopes. Positively charged ions and negatively charged ions can be divided into two classes based on the ion's charge.

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calculate the cross sectional area occupied by the nucleus as a fraction of that occupied by the atom. assume that the gold nuclei in each layer are offset from each other

Answers

For cross sectional area the area occupied by nucleus is 29.01 * 3.16227766e-7.

Cross sectional area is simply πr^2 to be scattered. Let us define this as two at a large angle.

Now define this as the substance noticeable to the nucleus. So if it's two atoms wide, then we required to take hold of the ratio of the cross sectional areas and then multiply it by two. As because of two layers we are hitting the nucleus twice. So now the diameter is divided by two.

Now ,

Particles to be scattered πr^2 / πr^2 = fraction

Radius and mass of nucleus = 3.04 * 10^-15 m,  3.04 * 10^ -23 g

r (nucleus) = 3.04*10^-15 m * 100 cm /1m

= 3.04 * 10^-13 cm

Area = πr^2

= 3.14* 3.04* 3.04* 3.16227766e-7

= 29.01 * 3.16227766e-7

In Rutherford's experiment for a two atom wide layer being scattered.

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Why does ether or petrol have a cooling effect when it touches the hand. Just curious​

Answers

During evaporation, particles of the liquid absorb energy from the surface of the palm to compensate for the loss of energy, making the surroundings cool. The acetone, petrol or perfume take this latent heat of vaporisation from our palm. The palm loses heat and feels cold.

Answer:

When we put some acetone or petrol or perfume on our palm, it evaporates. During evaporation, particles of the liquid absorb energy from the surface of the palm to compensate for the loss of energy, making the surroundings cool. This results in the palm becoming colder.

Explanation:

a particular brand of gasoline has a density of 0.737 g/ml at 25 ∘c . how many grams of this gasoline would fill a 13.5 gal tank ( 1us gal

Answers

Mass of gasoline = 37662.93 grams.

Density = mass/volume

mass = Density × volume

mass = 0.737 × 13.5 ×3785.41

mass = 37662.93 g

What is gasoline ?

The word "gasoline" is an American term for motor vehicle fuel. The trademark "Cazeline" or "Gazeline," which bears the last name of British publisher, coffee trader, and social activist John Cassell, is claimed to have had some impact on the phrase.

Gasoline, usually spelled gasolene, is a blend of flammable, volatile liquid hydrocarbons made from petroleum that is used as internal combustion engine fuel.

Numerous distinct compounds containing hydrogen and carbon make up gasoline (hydrocarbons). About 150 distinct hydrocarbons, including butane, pentane, isopentane, and the BTEX chemicals, are often included in a normal gasoline combination (benzene, ethylbenzene, toluene, and xylenes).

Crude oil, which comprises hydrocarbons, organic compounds formed completely of hydrogen and carbon atoms, is the raw material used to make gasoline.

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rank the carbonyl‑containing compounds in order of reactivity towards nucleophilic attack. you are currently in a ranking module. turn off browse mode or quick nav, tab to move, space or enter to pick up, tab to move items between bins, arrow keys to change the order of items, space or enter to drop. most reactive least reactive answer bank

Answers

From more reactive to least reactive carbonyl‑containing compounds :

3-methylpentanoyl chloride >benzoic anhydride   >   

butyl acetate> propionamide                                             

The carbonyl carbon should be more electron deficient to increase its reactivity for nucleophilic addition. The presence of R or Ar groups reduces this electron shortage. Less reactivity occurs when more of these groups are present.

The capacity of the electronegative leaving group to activate the carbonyl is connected to the relative reactivity of carboxylic acid derivatives toward nucleophile replacements. The carbonyl loses electron density as a result of the additional electronegative leaving groups, which also increases the carbonyl's electrophilicity.

The reactivity order of carbonyl compounds from more reactive to least reactive  :

acyl halide, acid anhydride, aldehyde, ketone, ester, carboxylic acid, amide, carboxylate ion

Benzoyl chloride, acetaldehyde, butyl acetate, and acetic anhydride are more reactive than acid chlorides and anhydrides. Ketones and aldehydes have lower reactivity.

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Describe two ways that can be used to identify which layer is organic and which layer is the aqueous layer in a liquid-liquid extraction.

Answers

Two ways that can be used to identify which layer is organic and which layer is the aqueous layer in a liquid-liquid extraction are:

There are many methods that can be used to distinguish two layers from each other using contrast between the two substances in certain properties. One of the common methods that can be used to distinguish aqueous layer from an organic layer is adding water to mixture and observing which layer increases in size. The layer that increases in the size is the aqueous layer, as water is immiscible with organic layer. Thus, water can only increase aqueous layer. Another way is, for a start, the ethereal layer, even laden with organic solute, should be less dense than aqueous layer, and thus should be floating on top. Sometimes you can even tell by rubbing solvent with your fingers.

What is extraction?

Extraction is a process used in organic chemistry for isolating a certain substance or a compound from its source material. Since the source material is composed of the various molecules and compounds but only a specific compound is needed, the desired compound has to be separated from the mixture.

A liquid-liquid extraction method is a method used to extract substance using the two immiscible of different natures. Since two liquids are immiscible, they liquids form the two layers when added to the same vessel. This is typically done using the water and an organic solvent that is insoluble in water. When two are mixed, they form an aqueous layer and an organic layer.

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i need help with this question

Answers

Answer:

here is your help..

Explanation:

you add 57 and 23 then round the  number to the highest

One of the two amino acids that has a side chain including the modified carboxyl group, carboxamide, is _______________.

Answers

One of the two amino acids that has a side chain including the modified carboxyl group, carboxamide, is Asparagine.

Non-essential amino acid aspartic acid (Asp/D) has a carboxyl group in its Rgroup. Oxaloacetate is transaminase to easily create it. At physiological pH, the side chain of aspartic acid has a pKa of 3.9 and is negatively charged.

The side chain of the amino acid cysteine is chemically reactive and capable of forming bonds with other cysteines.

At this location, known as the side chain, each amino acid is joined to a different chemical group. Each amino acid differs from the others due to its side chain, which also gives it a distinctive set of chemical properties. The side chain is frequently referred to as a R group and is represented by the letter R.

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if 34.4 grams of pentane (c5h12) are burned in excess oxygen, how many grams of h2o will be produced?

Answers

If 34.4 grams of pentane ( C₅H₁₂) is burned in excess oxygen, 51.6 grams of H₂O will be produced.

The following reaction occurs when pentane is burned in the presence of excess oxygen,

C₅H₁₂ + 8O₂ = 5CO₂ + 6H₂O

We can observe from the above reaction that,

1 mole of C₅H₁₂ produces 6 moles of H₂O.

Hence,

72 g of C₅H₁₂ produces 108 g of H₂O

1 g of C₅H₁₂ produces 108/72 g of H₂O

34.4 g of C₅H₁₂ produces 108/72 x 34.4 g = 51.6 g of H₂O.

Therefore, if 34.4 grams of pentane ( C₅H₁₂) is burned in excess oxygen, 51.6 grams of H₂O will be produced.

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i need the answers pleaseee! this is acids and bases for chemistry

Answers

Baking soda is a weak base

molecule ""a"" is diatomic and has double bonds. the bond length between the atoms is 0.5 nm. what would be your guess about the van der waal’s radii for the two atoms present in this molecule?

Answers

Molecule "a" is  O2

The O2 Lewis structure has a double bond between two oxygen atoms.  consistent with the octet rule, oxygen atoms have to bond twice. The O2 molecule is diatomic, meaning that two atoms of identical elements are connected in a pair.

What is an octet rule?

The octet rule may be a  chemical rule of thumb that reflects the theory that main-group elements tend to bond in such a way that each atom has eight electrons in its valence shell, giving it the identical electronic configuration to a noble gas.

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At what temperature will water change from a liquid to a solid (freeze)?

Answers

Answer:

Water changes from liquid to a solid at 32 degrees Fahrenheit

Answer:

32°F

Explanation:

explain 2 ways how there can be forces acting on an object yet the object remains at rest

Answers

There can not be two forces acting on a object that is simply impossible

Given that the charge on an electron is 1.60×10−19 C , determine the conversion factor between electrinos and coulombs.

Answers

The conversion factor from electrino to coulombs is 1.60×10−19.

What is charge?

The term charge refers to a particle that is positively or negatively charged. The electron is a particle that is negatively charged. One of the popular units that could be used when dealing with electrons is the electrinos.

We know that;

1 electrino = 1.60×10−19 C

It then follows that the conversion factor from electrino to coulombs is 1.60×10−19.

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The molecular weight of this compound was determined by mass spectrometry to be 264 g/mol. what is the molecular formula of this compound?

Answers

The Empirical Formula for the given compound is C₃H₄O₃ .

What is an Empirical Formula?The simplest whole number ratio of atoms in a chemical molecule is its empirical formula in chemistry.As a result, the empirical formula for sulfur monoxide and disulfur dioxide, two compounds made of sulfur and oxygen, is the same.Their molecular formulae, which represent how many atoms are present in each molecule of a chemical compound, are different.The number of atoms or their arrangement is not mentioned in an empirical formula.The number of each sort of atom in a molecule is displayed in the molecular formula, though.The molecule's organization is represented by the structural formula. Additionally, different types of compounds may have empirical formulas that are equivalent.

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Using the information below, find how many minutes are in 0.56 yrs.
1 day = 24 hrs
1 yr = 365 days
1 hr = 60 min

Answers

There are 294,336  minutes are in 0.56 yrs.

Minutes are a reputable recording of the court cases of a Board meeting Annual fashionable meeting or another meeting and the enterprise transacted on the meeting.

1 yr = 365 days

0.56 = 365 × 0.56

       = 204.4 days.

1 day = 24 hrs

204.4 days = 204.4 × 24

                  = 4905.6 hours

1 hr = 60 min

4905.6 hours =   4905.6 × 60

                    = 294,336 minutes.  

All agencies registered in India are required to maintain mins of all Board and Committee conferences in a minutes e-book. minutes may be created during the meeting with the aid of a typist or courtroom reporter, who might also use shorthand notation and then prepare the minutes and problem them to the contributors afterward.

Minutes are a tangible report of the meeting for its contributors and a source of facts for members who had been not able to wait. In a few cases, assembly mins can act as a reference point, as the instance when a meeting's outcomes affect different collaborative activities or initiatives inside the corporation

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compare the three characteristics of the states of matter ( solid , liquid and gas)

you answer and i will mark u as brainliest

Answers

Explanation:

btw this is secondary school science level :

solid:

•solids have a definite shape and volume.

• they cannot be compressed

•high density

•particles vibrate in a fixed position

•high melting point

Liquid:

•no fixed shape

•partcles are free to move over eachother but are still attracted to one another

•almost incompressible

•have fixed volume but no fixed shape

•denser tagt solids

•there is space between particles

Gas:

•easy to compress

•expand to fill their containers

•particles are free to move around

•Do not have a fixed volume or shape

•gasses can diffuse- their particles move from an are of high concentration to low concentration

•low density

these are just some things of the top of my head. hope it helps

annie learns that the compound sulfuric acid is called the universal chemical. the acid is used in everything from gasoline to paper. annie uses beads in three different colors to model sulfuric acid. based on the model, what is the chemical formula of sulfuric acid?

Answers

The compound Annie learns about that is sulfuric acid which is the universal solvent has a chemical formula of H2SO4.

What is the chemical formula of a compound?

The chemical formula of a substance serves as a representation of that substance's chemical make-up. The elements that make up a compound's molecules and the ratio in which their atoms combine to produce those molecules are described in a chemical formula.

The compositions of chemical compounds can be stated in a variety of ways, even though the phrase "chemical formula" is typically used to refer to the molecular formula of a compound, which represents the total number of atoms of each constituent element in one molecule of the compound.

Thus, the compound Annie learns about that is sulfuric acid which is the universal solvent has a chemical formula of H2SO4.

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The table shows the substances used during a science experiment. Which substances are elements?HELP ME PLS!

Answers

Answer:What are 10 elements examples?

Examples

H - Hydrogen.

He - Helium.

Li - Lithium.

Be - Beryllium.

B - Boron.

C - Carbon.

N - Nitrogen.

O - Oxygen.

Explanation:

The lowest portion of the gi tract, where water and electrolytes are absorbed and waste is eliminated, is the __________.

Answers

The lowest portion of the GI tract, where water and electrolytes are absorbed and waste removed , is the    Large Intestine

What are gastrointestinal diseases?

Gastrointestinal diseases affect the gastrointestinal (GI) tract from the mouth to the anus. They are of  two types basically : functional and structural. Some examples include nausea/vomiting, gastrointestinal disorder , lactase deficiency and diarrhea.

What is the GI tract responsible for?

The gastrointestinal system is consist of the gastrointestinal (GI) tract, or the alimentary tract , salivary glands, the liver, and therefore the exocrine pancreas. The  main functions of the alimentary canal are to digest and absorb ingested nutrients, and to excrete waste materials  of digestion.

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