What happened if we add normal saline instead of PBS during beta amylase extraction?​

Answers

Answer 1

Answer:

hi

Explanation:

Answer 2

Saline is the solution of water and NaCl does not provide the appropriate pH, so enzyme activity is lost, we cannot add saline instead of PBS during beta amylase extraction.

How saline water affects beta amylase extraction?

PBS (phosphate buffer solution) is a type of buffer that is suited for beta-amylase activity, which implies that when beta-amylase is extracted using buffer, the enzyme's chemical activity is kept.

When we utilize saline, which is a solution of water and NaCl, the pH of the solution is not acceptable, and the enzymes' chemical activity is destroyed.

As a result, for enzyme/protein extraction, we always employ a buffer solution, which maintains the ph for the proper functioning of an enzyme.

Therefore due to pH conditions enzyme lost activity, and normal saline water can not be used instead of PBS for extraction of beta-amylase.

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Related Questions

Answer this please. Its gizmos

Answers

the answer is A. because none of the other answers allowed for a full white AND a full red chicken

When blood is drawn from your arm, what type of vessel does the phlebotomist use? Why?

Answers

Answer:

1. Median cubital vein A superficial vein, most commonly used for venipuncture, it lies over the cubital fossa and serves as an anastomosis between the cephalic and basilic veins. 2. Cephalic vein Shown in both forearm and arm, it can be followed proximally where it empties into the axillary vein.

Explanation:

True or false Solutions of equal solute concentration are isotonic.

Answers

Answer:

Explanation:

now you have two mixtures of different solute concentrations. In comparing two solutions of unequal solute concentration, the solution with the higher solute concentration is hypertonic, and the solution with the lower solute concentration is hypotonic. Solutions of equal solute concentration are isotonic.

What's a chromosome

Answers

A chromosome is a strand of DNA that is encoded with genes. In most cells, humans have 22 pairs of these chromosomes plus the two sex chromosomes (XX in females and XY in males) for a total of 46.

A sample of a meteorite has a density of 23 g/ml. It takes up a volume of 147 ml.
What is the mass of this unknown sample?

Answers

Answer:

3,381 grams

Explanation:

The problem can be solved by using the formula for "Density." The formula for density is: Density (ρ) = [tex]\frac{mass}{Volume}[/tex]

Now that we know the formula, we can get the formula for the "mass."

mass = ρ x Volume

The given density above is 23 g/mL while the given volume is 147 mL. We have the values of the density and volume, thus, it is easy to solve the problem.

Let's solve.

mass = 23 g/mL x 147 mLmass = 3,381 grams

The mass of the meteorite is 3,381 grams.

What would happen if a cell couldn't make catalase?

Answers

Answer:

If hydrogen peroxide is not broken down by catalase, additional reactions convert it into compounds called reactive oxygen species that can damage DNA, proteins, and cell membranes. ... A shortage of this enzyme can allow hydrogen peroxide to build up to toxic levels in certain cells

If hydrogen peroxide is not broken down by catalase, it can be converted into reactive oxygen species, which can damage DNA, proteins, and cell membranes.

What is catalase?Catalase is an important enzyme that uses hydrogen peroxide, a non-radical ROS, as a substrate.This enzyme breaks down and neutralizes hydrogen peroxide to maintain optimal molecular levels within cells.It is also essential for cell signaling processes. The catalase enzyme breaks down hydrogen peroxide (H2O2) into water and molecular oxygen, protecting cells from oxidative damage by reactive oxygen species.Commercially available catalase is produced from Aspergillus niger by a solid-state fermentation process.Peroxidase or catalase is an enzyme of the oxidoreductase class. The peroxidase enzyme catalyzes the breakdown of hydrogen peroxide into water and molecular oxygen as shown.Catalase is a tetrameric heme protein that detoxifies H2O2 into oxygen and water.It is a metalloprotein oxidoreductase and when present in high concentrations he efficiently removes H2O2.

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What did scientists believe was contributing to the depletion of the ozone layer and why?

Answers

The ozone is a layer in the stratosphere which protects the earth from ultraviolet light. The molecule within the ozone are being broken apart due to fossil fuel emissions.

CFCs and other halogen-source chemicals in the stratosphere were discovered to deplete the ozone layer.

What is ozone layer depletion?

Ozone depletion is the steady lowering of the Earth's ozone layer in the high atmosphere, which is produced by the discharge of chemical compounds from industry and other human activities that contain gaseous chlorine or bromine.

The ozone layer acts as a buffer against the sun's potentially dangerous ultraviolet rays. The destruction of the ozone layer puts humans in direct contact with dangerous ultraviolet rays, which can lead to a variety of health problems including cancer, cataracts, skin illnesses, and a weakened immune system.

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How have protozoa typically been classified?

by their type of reproduction

by their symmetry

by their mode of locomotion

by their mode of nutrition

Answers

Answer:

by their mode of locomotion

Explanation:

I'm not too sure but.. :)

What do plants produce during photosynthesis? F oxygen G light H magnetic energy J carbon dioxide

Answers

Answer:

yes

Explanation:

PLS HELP ME ON THIS ONE TOO!!!:))

Answers

Answer:

it is c on 1 D on 4 A on 2 and Bon 3

why do the other planets take longer to orbit the Sun than the inner planets do​

Answers

Answer:

Outer planets take longer to orbit than inner planets because of the greater distance they need to cover. They also are further from the sun weakening the power of the suns gravitational pull which causes then to orbit slower.

Explanation:

!!

Answer:

Because there are outter planets

Explanation:

Three cells that each has a diploid number of 32 go through meiosis. How
many cells result and how many total chromosomes are in each cell?

Answers

Answer: 6 cells, 16 chromosomes each.

Explanation:each cell, after meiosis produces two haploid cells.

three diploid cells (2n=32) after meiosis give six haploid cells

If you’re good at this could you help me please?

Answers

Answer:

I'm pretty sure its D or B

On Isle Royale, wolves are the main predators of moose. The graph shows the changing wolf and moose populations on the island over a 50-year time span.

The graph plots the populations of moose and wolves in separate lines from 1955 to 2005. The two populations appear to follow opposite trends: when one population is higher, the other population is lower.

Which statement provides the strongest explanation for the trends in the two populations?

A. The populations of predators and prey are limited by abiotic factors only.
B. Predation acts a density-dependent limiting factor on prey, but not on predators.
C. Predation acts as a density-independent limiting factor on predators, but not on prey.
D. Predator-prey relationships act as density-dependent limiting factors on both predators and prey.

Answers

Answer:

D

Explanation:

The statement provides the strongest explanation for the trends in the two populations is predator-prey relationships act as density-dependent limiting factors on both predators and prey. Hence option D is correct.

What is predator?

Predator is defined as an organism that mostly hunts and consumes other organisms for food. The term "predator" refers to a species that hunts and eats specific other organisms. The animals that predators consume are referred to as prey.

Predator-prey interactions and prey-predator interactions are both crucial density-dependent population controls. The other population's size determines how each population changes in size.   When the population density reaches a particular point, a density-dependent limiting factor takes effect. Competition, parasitism, and disease are other instances of density-dependent limiting variables.

Thus, the statement provides the strongest explanation for the trends in the two populations is predator-prey relationships act as density-dependent limiting factors on both predators and prey. Hence option D is correct.

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October 1, 2017 marks 27 years since the beginning of the Human Genome
Project. The goal of the group was to map the entire 3 billions bases and
80,000 genes in the human genome. On April 14, 2003, it was announced
that the goal was accomplished. Using this information, scientists have been
able to predict the risk of genetic diseases and understand the process of
inheritance. Which of the following statements best describes the process of
using the genetic code to produce polypeptides (proteins)?
Translation is used to produce a strand of RNA and proteins from a single strand of DNA.
O RNA produces a strand of DNA through transcription, then the DNA builds a protein molecule
through translation
O A single strand of DNA is used to make mRNA using translation, then the mRNA provides the
template for producing a protein through transcription
O A single strand of DNA is used to produce mRNA through transcription, then translation provides
the code for bonding amino acids to make a protein.

Answers

Answer:

The last one. A single strand of DNA is used to produce mRNA through transcription, then translation provides the code for bonding amino acids to make a protein.

Explanation:

Educated guess, but also. Translation is the process by which mRNA is decoded and translated to produce a polypeptide sequence, otherwise known as a protein. This method of synthesizing proteins is directed by the mRNA and accomplished with the help of a ribosome, a large complex of ribosomal RNAs (rRNAs) and proteins.

What are the signs and symptoms of sickle cell anemia?

Answers

symptoms

Explanation:

swelling

frequent infections

delayed growth or puberty

vision problems

sickle cells break apart easily and die leaving you with to few red blood cells

Symptoms
Anemia. Sickle cells break apart easily and die, leaving you with too few red blood cells. ...
Episodes of pain. Periodic episodes of pain, called pain crises, are a major symptom of sickle cell anemia. ...
Swelling of hands and feet. ...
Frequent infections. ...
Delayed growth or puberty. ...
Vision problems.

The membrane is stiffened in some spots, decreasing its fluidity, by the process of _______ molecules

Answers

Phospholipids Molecules

Explain how the biogeochemical cycle are essential for life.​

Answers

Biogeochemical cycles are essential for life and important to the ecosystems because matter on earth is limited in amount, and space for dead organisms as well. Additionally, biogeochemical cycles are important as as they transport and store these chemical elements so that they can be used by living organisms.

Because Earth has limited materials and room for deceased creatures, biogeochemical cycles are vital to life and ecosystems. Biogeochemical cycles transport and store these chemicals for living creatures.

What are biogeochemical cycle?

Because there is a finite quantity of stuff on earth and a certain amount of room for dead species as well, biogeochemical cycles are crucial for life and significant to ecosystems. Additionally, biogeochemical cycles are crucial because they move and store these chemicals so that living things may use them.

The flow of nutrients and other elements between biotic and abiotic forces is referred to as "biogeochemical cycles" in most cases. The words "bio" and "geo," which refer to the biosphere, "geo" and "chemical," which refer to the elements that flow through a cycle, are the roots of the phrase "biogeochemical."

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3. Which of the following is a chemical property
(10 Points)
melting point
Ostates of matter
density
flammability
4. Substances formed as a result of a chemical reaction are called *

Answers

Answer: The answer is flammability

Explanation:

because it is a chemical thing happening

The normal pulse rate is ___________. *
1. 70 to 90 per minute
2. 70 to 80 per minute
3. 40 to 70 per minute
4. 70 to 100 per minute
Class 7

Answers

A normal pulse rate is 70 to 100 per minute

C. 70 to 100 per minute

7. How do the data support your claim above?
Explain your statement above. Be sure to refer to specific pieces of data from your experiment that
support your argument. HELP

Answers

Well what’s the data

Which of the following best explains the endosymbiosis theory of how eukaryotic cells originated, as shown below? A.Eukaryotic cells developed from mutations in prokaryotic cells
B.Eukaryotic cells developed from organic compounds carried to Earth by debris from space
C.Eukaryotic cells originated from a combination of gases and heat in the atmosphere
D. Eukaryotic cells were originally large prokaryotic cells that absorbed smaller prokaryotic cells

Answers

Answer:I’m sure the answer is D

Explanation:

All eukaryotes evolved from prokaryotes and eventually developed into mitochondrial organelles.

The endosymbiotic theory represents that some of the organelles in eukaryotic cells were prokaryotic microbes at some time. As per the endosymbiosis theory the eukaryotic cells were at first large prokaryotic cells that absorbed smaller prokaryotic cells.

What is endosymbiosis?

Endosymbiosis is the engulfing of the cell by another one to produce, over time, a coevolved relationship in which none of the cell could survive alone.

It is an evolutionary theory that guides the origin of eukaryotic cells by prokaryotes.

Eukaryotic cells were previously large prokaryotic cells that actually absorbed smaller prokaryotic cells

Thus, the correct option is D.

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How much ATP is produced in 5 molecules of glucose in the presence of oxygen in an obligate aerobe?

Answers

Answer:

190 ATP molecules

Explanation:

Cellular respiration is the process whereby energy (ATP) is obtained in the cells of living organisms by breaking down glucose molecules. Cellular respiration can either be aerobic or anaerobic depending on whether oxygen is present or not.

In the presence of oxygen, aerobic respiration, which consists of three stages namely: glycolysis, Krebs cycle and oxidative phosphorylation, is employed. Per glucose molecule;

- Glycolysis produces 2 ATP molecules,

- Krebs cycle produces 2 ATP molecules

- Oxidative phosphorylation produces 34 ATP molecules

Hence, in total, 38 ATP molecules is produced per glucose molecule in aerobic respiration. According to the question, 5 molecules of glucose will yield 5 × 38 = 190 molecules of ATP.

If the endoplasmic reticulum were removed from the cell, which organelle would not be able to function properly, and why? mitochondria, because they rely on the ER as an energy source ribosomes, because they receive proteins made by the ER Golgi apparatus, because it packages proteins received from the ER cytoplasm, because it relies on the ER for structural support

Answers

Answer:

Golgi apparatus, because it packages proteins received from the ER

Explanation:

The function of the endoplasmic reticulum is to produce proteins. It can be smooth or rough. The rough endoplasmic reticulum has ribosomes, which make the proteins.

The Golgi apparatus receives proteins from the endoplasmic reticulum and modifies and sorts them. They are then concentrated and packed into vesicles which are released out of the cell membrane.

If the endoplasmic reticulum is removed, proteins cannot be made and the Golgi apparatus would not be able to perform its job.

Hope that helps.

If the endoplasmic reticulum (ER) were removed from the cell, Golgi apparatus would not be able to function properly.

What is endoplasmic reticulum?"Endoplasmic Reticulum is a network of membranous tubules present within the cytoplasm of a eukaryotic cell."It is of two types- Smooth Endoplasmic Reticulum and Rough Endoplasmic Reticulum.It helps in calcium storage, protein synthesis and lipid metabolism.What is Golgi apparatus?It is also known as Golgi body or Golgi complex.It helps in transporting, modifying and packaging of proteins and lips lipids which are synthesized by Endoplasmic Reticulum.

Hence, Golgi apparatus would not function properly if Endoplasmic Reticulum is removed.

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Use the context of the urinary system to explain the term osmoregulation

Answers

Answer:Kidneys regulate the osmotic pressure of a mammal's blood through extensive filtration and purification, in a process known as osmoregulation. Kidneys filter the blood; urine is the filtrate that eliminates waste from the body via the ureter into the bladder.

Explanation:

Kidneys regulate the osmotic pressure of a mammal's blood through extensive filtration and purification, in a process known as osmoregulation. Kidneys filter the blood; urine is the filtrate that eliminates waste from the body via the ureter into the bladder.

what are the differece between facts theory and hypothesis in scientific ex'p​

Answers

Answer:

Theory, and Law In Science.

Explanation:

for a phenomenon made as a starting point for further investigation.

please help my little sibling fr needs it . thank u !

Answers

Answer:

1 is C

2 is A

3 is B

Explanation:

1 - the cow is breathing and releases carbon dioxide the tree absorbs it and releases oxygen

2 - the cow Is breathing that's why it releases carbon dioxide

3 - the human takes in oxygen but breaths out carbon dioxide

Genetics Problem Solving Chapter 25

Answers

Answer:

See the answer below

Explanation:

1.      Tt   x   Tt

   TT  Tt   Tt   tt

Assuming that T is dominant over t:

Genotype ratio = 1TT:2Tt:1tt

Phenotype ratio = 3:1

2.     Rr   x   Rr

    RR   Rr   Rr   rr

Assuming that R is dominant over r:

Genotype ratio = 1RR:2Rr:1rr

Phenotype ratio = 3:1

3.  Assuming that green (G) is dominant over the other color (g). Heterozygous green would be Gg

        Gg   x   Gg

     GG   Gg   Gg   gg

Genotype ratio = 1GG:2Gg:1gg

Phenotype ratio = 3:1 (green ratio the other color)

4. Assuming that green (G) is dominant over the other color (g) as above:

  homzygous green plant = GG; heterozygous green plant - Gg

      GG   x   Gg

   GG   Gg   GG   Gg

Genotype ratio = 1GG:1Gg

Phenotype ratio = 100% green

5. Assuming that tall (T) is dominant over short (t)

Homzygous tall plant = TT

short plant = tt

         TT   x   tt

       Tt   Tt   Tt   Tt

Genotype ratio = 100% Tt

Phenotype ratio = 100% tall

What are the PRODUCTS (what is made) of aerobic respiration? Select all that apply

Answers

Answer:

carbon dioxide and water

In the United States, it is possible for discoverers of gene sequences in organisms to patent those sequences. This gives the discoverer property rights for the sequence. Many scientists object to this practice. Which is the strongest scientific argument against allowing a gene sequence to be patented?

Answers

Answer:Are gene sequences patentable under existing law?

Explanation:

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