Restriction endonucleases cleave at __________ , which are sequences that read the same, 5 to 3 , on each DNA strand.

Answers

Answer 1

Answer:

Restriction endonucleases cleave at  DNA (Deoxy ribonucleic acid) which are sequences that read the same, 5 to 3 , on each DNA strand.

Explanation:

RESTRICTION ENDONUCLEASES-: An enzyme that cleaves DNA into fragments at or near unique recognition sites within molecules known as restriction sites is a restriction enzyme, constraint endonuclease, or restrictase. One class of the larger endonuclease group of enzymes is restriction enzymes. Restriction enzymes are typically divided into five groups, which vary in structure and whether their DNA substrate is cut at their location of recognition or whether the locations of recognition and cleavage are different from one another. Both restriction enzymes make two incisions in order to cut DNA, once through each sugar-phosphate backbone (i.e. each strand) of the double helix DNA.

In bacteria and archaea, these enzymes are contained and provide a defence mechanism against invading viruses. Within a prokaryote, in a process called restriction digestion, the restriction enzymes selectively cut up foreign DNA; meanwhile, host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two procedures form the method of restriction alteration.


Related Questions

In simple dominance, the dominant trait
a. is masked by the recessive trait
b. masks the recessive trait

Answers

Answer:

b. maks the recessive trait

Quick explanation:

The dominant trait masks the reccessive gene, but the person still has the gene, it just won't affect them, it might affect their future generations. For example, the trait for blue eyes is a recessive trait, so in order for you to have blue eyes, both parents will need to have the gene for blue eyes.

The correct answer is b!

True or False: A is the hanging wall and B is the foot wall.

Answers

Answer:

False

Explanation:

so they hanging walls above the plane of the fault (and over your head and the footwall is below the plane of the fault (and is under your feet)

The buring of fossil fuels releases which of the following gases into the atmosphere

Answers

what are the choices?

DNA sequences can act as "tape measures of evolution". Scientists analyzing the human genome sequence were surprised to find that some of the regions of the human genome that are most highly conserved (similar to comparable regions in other species) don't code for proteins. What is a possible explanation for this observation? EVOLUTION CONNECTION sequences can act as "tape measures of evolution". Scientists analyzing the human genome sequence were surprised to find that some of the regions of the human genome that are most highly conserved (similar to comparable regions in other species) don't code for proteins. What is a possible explanation for this observation?

Answers

Answer:

Non-coding DNA regions play important roles in regulating transcriptional activity by encoding different types of non-coding RNAs (ncRNAs), acting as scaffold attachment regions, acting as enhancer specific regions, etc.

Explanation:

Historically, it had been believed that non-coding DNA sequences were 'junk DNA' since they don't encode for proteins (beyond the sequences that are transcribed into functional non-coding RNAs, i.e., transfer RNA and ribosomal RNA). However, in the last years, it has been shown that non-coding DNA sequences play critical roles in regulating gene expression and genome function. For example, evolutionary conserved non-coding RNAs (ncRNAs) with regulatory roles on gene expression such as, for example, long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) have been mapped in non-coding DNA sequences, thereby evidencing the functional significance of these regions. In consequence, the conservative nature of certain non-coding DNA sequences evidence that mutations in such regions may have significant deleterious effects, and thereby they could have a negative impact on the fitness of the individual.

Plato's allegory of the cave indicates that

majority opinion is almost always correct.

truths about reality are not always obvious.

when we seek the truth, we must always trust our senses.

imagination is a more reliable guide than objective reality?

Answers

Answer:

truths about reality are not always obvious.

Explanation:

Plato's allegory of the cave was proposed by the Greek philosopher called Plato. This was used to emphasize the importance of education and the effect caused by a lack of it.

Plato's allegory of the cave indicates that truths about reality are not always obvious. This implies that no matter how real something seems to be it is always ideal to ask questions about it which is useful in problem solving.


Match the following:
A
1. Relative humidity
2. Rainfall
3. Wind speed
4. Temperature
5. Atmospheric pressure
B
•millibars
• kilometres per hour
• degree Celsius
• per cent
• centimetres​

Answers

Answer:

को 'वीर सिपाही' क्यों कहा

In which biome would you find the highest biodiversity?
A)
grassland
B)
tundra
C)
savanna
D)
deciduous forest
E)
rainforest

Answers

It depends on what context, but I would say

E) Rainforest.

Due to the fact that it’s the ideal temperature for many organisms

What can you conclude about the advantage of a double layer arrangement of a cell membrane

Answers

Answer:

The cell requires a barrier between itself and its environment.

Explanation:

hope I helped you let me know if u need anything :)

What does this diagram represent?

Answers

It represents DNA since it is double stranded.

he percentage of recombinant individuals that Morgan noted while examining X-linked traits was thought to indicate... Select an answer and submit. For keyboard navigation, use the up/down arrow keys to select an answer. a the relative distance between the genes b the presence of more than two alleles at each locus c that genetic material was being exchanged with the Y chromosome d the number of genes that could be found on any given chromosome

Answers

Answer:

The correct option is a) the relative distance between the genes.

Explanation:

A short distance between genes is a very little target for crossing-over to occur, which means that only a few of them will happen, compared with the number of events between genes that are more separated between each other.  

Two genes that are very close have a few recombination events and are strongly bounded. These genes are linked.

The percentage of recombination frequency (P = Recombinant number / Total of individuals) is useful to determine the genetic distance between two or more genes. This distance results from multiplying that frequency by 100 and expressing it in map units (MU) or centiMorgans (cM). One centiMorgan (cM) equals one map unit (MU).  

The map unit or centiMorgan is the distance between the pair of genes for which every 100 meiotic products only one results in a recombinant product.  

Why does the amount of energy available change as you move from one
trophic level to the next? Does this process still follow the Law of
Conservation of Energy? Explain your reasoning.

Answers

Answer:

hope it helps you

Explanation:

Energy decreases as it moves up trophic levels because energy is lost as metabolic heat when the organisms from one trophic level are consumed by organisms from the next level. Trophic level transfer efficiency (TLTE) measures the amount of energy that is transferred between trophic levels.

life in desert biomes tend to be limited by

Answers

Answer:

Low amounts of water

Explanation:

Deserts mean areas with low amounts of water and animals are not able to survive without water therefore  not having water is a limiting factor

(Limiting factors can also be said as limited by)

what solution describes a saturated solution?

Answers

Answer:

This is not my answer, I found it on the internet.

No more solutes can be dissolved in a saturated solution at a given temperature and pressure.

Explanation:

A saturated solution is a solution that consists of maximum amount and concentration of the solute that is dissolved in the solvent.

The extra amount of solute cannot be dissolved in a saturated solution.

The saturated solution is composed of solute and solvent and solvent cannot dissolve more solute at some extent.

The carbonated beverage is an ideal example of saturated solution.

A saturated solution is one that contains the greatest concentration and amount of the solute dissolved in the solvent. In a saturated solution, the excess solute cannot dissolve.

What is Saturated solution?

Solvent and solute make up the saturated solution, and to a certain extent, solvent cannot dissolve more solute. A saturated solution is best exemplified by fizzy beverages.

A solute's solubility is the greatest amount of that solute that can dissolve in a solvent at a given temperature and pressure. The moles of solute per volume (mol/L) or the mass of solute per mass of solvent (g/g) are other common ways to express solubility.

There is typically a limit to how much solute can dissolve in a given amount of solvent, even for extremely soluble compounds. In general, temperature and pressure—for gases—as well as the energy components we have already covered affect a substance's solubility.

Therefore, A saturated solution is one that contains the greatest concentration and amount of the solute dissolved in the solvent. In a saturated solution, the excess solute cannot dissolve.

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Which word is a synonym for Heterotroph?
Group of answer choices

A)Consumer

B)Monster

C)Bacteria

D)Producer

Answers

A is the answer. A heterotrophs is an organism that consumes other organisms so it is a consumer!

why do neutral elements form ions ?

Answers

Answer:

because they can be atoms

Explanation:

and they can also remove one or more electrons at a negatively charged.

Which of the following is true regarding the machinery of translation?

a. A single mRNA can be translated simultaneously by several ribosomes.
b. tRNAs released from the ribosome are degraded B) termination and eukaryotes utilizes three different release factors.
c. Polycistronic MRNA usually has a single ribosome binding site.
e. once a single mRNA strand is translated once it is degraded

Answers

Answer:

The correct answer is: a. A single mRNA can be translated simultaneously by several ribosomes.

Explanation:

Ribosomes are structures composed of ribosomal RNA (rRNA) that direct the process of translation, with the help of special proteins and key molecules called transfer RNA (tRNA) that are 'able to read' the codons in the mRNA. To help with this 'reading', ribosomes move through the mRNA strand as the translation moves forward, placing the anticodons presented by the tRNA molecules with their specific match - each mRNA codon - forming a strand of amino acids.

To ease the process in the cases where the molecule to be translated is a polypeptide, groups of ribosomes form a polysome and they all translate one single mRNA strand at the same time. Each of these ribosomes starts translating from the first codon and stop when the stop codon appears.

The true statement regarding the machinery of translation is ; ( A ) A single mRNA can be translated simultaneously by several ribosomes

Translation is the process of producing protein with the use of RNA ( i.e. the process of protein synthesis from  an mRNA template )  during translation the code found in the template is converted into an amino acid sequence.

Ribosomes are composed of rRNA which directs the process of translation with the proteins and key molecules such as tRNA.

Hence we can conclude that the true statement regarding the machinery of translation is A single mRNA can be translated simultaneously by several ribosomes.

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Select all of the following that describe RNA.
Check all that apply.
nucleotides contain the sugar deoxyribose
always occurs as a double-stranded helix
can be single-stranded
can catalyze chemical reactions
the nitrogenous base thymine is used instead of guanine
the nitrogenous base uracil is used instead of thymine
nucleotides contain the sugar ribose

Answers

Nucleotides contain the sugar ribose
Nucleotides contain the sugar deoxyribose
Can be single-stranded

The statements that describe RNA are ;

Nucleotides contain the sugar ribose ( 7 )Can be single stranded ( 3 )The nitrogenous base uracil is used instead of thymine ( 6 )

RNA and DNA are similar in some ways because they are both nucleic acids but the characteristics of RNA that makes it different from DNA is that RNA is mostly single stranded with it Nucleotides containing ribose instead of deoxyribose.

The RNA ( Ribonucleic acid )  nucleotides are composed of certain bases i.e. adenine, guanine, and cytosine also it contains uracil in place of thymine.

Hence we can conclude that the statements that describe RNA are ; Nucleotides contain the sugar ribose, Can be single stranded, The nitrogenous base uracil is used instead of thymine.

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Sea turtles spend most of their lives at sea feeding on jellyfish. When it is time to lay their eggs, mature females come ashore. They dig
nests in the sand along the coast, lay their eggs, and then return to the sea. The data below show the changing sea levels since the late
1800s.

Answers

Answer:

this is not a very clear question T-T

Explanation:

wish i could help

Answer:

Explanation:

Afterwards, only females come ashore to nest; males almost never return to land ... Most females return faithfully to the same beach each time they are ready to nest. ... When the turtle has finished digging the egg chamber, she begins to lay eggs. ... Well over 90% of a sea turtle's life is spent in the water – feeding, mating, ...

why do our digestive systems produce separate enzymes to digest proteins, fats, and carbohydrates?

Answers

Answer:

The digestive system produces different types of enzymes in order to convert proteins, fats and carbohydrates into smaller molecules and facilitate their absorption.

Explanation:

Once food is ingested, it must go through a process of digestion, which involves the conversion of food into smaller molecules, in order to facilitate its absorption at the intestinal level.

There are different enzymes, produced in the digestive system, for this purpose:

Proteases degrade meat proteins to convert them into amino acids. Lipases convert fats into fatty acids. Amylases convert sugars and carbohydrates into glucose.

The main objective is to obtain the fundamental components of each food to be assimilated by the organism, via intestinal absorption.

Here is what I want you to do. Create a comic strip using one of the following websites. Some of the websites you must pay for, so DON'T PAY for them, just take a screenshot and send it to me in an email.


You can also complete the project using poster board. Either way is fine. Once completed with poster board, you must send it to me by taking a picture through an email.

The key to the assignment is to make sure you will model the structures of the cell and describe their functions. You will do this by completing a table that describes the functions of structures of the cell. The table should also identify factory parts or workers that have similar functions.

The comic strip is just a way to describe the cell structures. It's a scenario (scene) describing the events. The scene is of a reporter who visits a cell “factory” and interviews someone about the structures/organelles and how their roles in the cell are similar to those of a factory and its workers.

Comic Strip Websites:

Answers

Answer:

I just did this test the correct one is B.

Docosahexaenoic acid (DHA) is an omega-3 fatty acid essential for brain development during pregnancy and early childhood. It is also linked to improved heart health, better vision, and reduced inflammatory response.

An energy pyramid shows the amount of available at each feeding level in an ecosystem.​

Answers

Answer:

An energy pyramid is a diagram that compares the energy used by producers, primary consumers, and other trophic levels. In other words, an energy pyramid shows how much energy is available at each trophic level. Energy is lost at each trophic level of a food chain.

Answer:

its energy

Explanation:

took the quiz

Genes A and B are neutral. A weakly beneficial mutation arises in the population. This mutation is 100 base pairs away from Gene A and 1000 base pairs away from Gene B. If this mutation were to go to fixation within the population, which gene would be more likely to go to fixation and what is the term for this process? Is there any reason to suspect that one or both of these genes may not go to fixation? Why or why not?

Answers

Answer:

Both genes would be likely to go to fixationThe term for this process is "linked genes"The reason to suspect that both of these genes may not go to fixation is that they are too close to the mutation and the recombination frequency between them is very very low.

Explanation:

Independent assortment law establishes that the alleles from two or more different genes distribute in gametes independently from each other. In other words, a gamete receives an allele from a gene that does not depend nor influence the allele of another gene in the same gamete. This can only be applied to independent genes. These genes segregate independently after crossing-over because they are located far away from each other.

Some other genes, however, are too close to each other and they do not segregate independently. These are the linked genes that do not exhibit an independent distribution, and they inherit together more frequently.  

Crossing-over between linked genes that are very close to each other in the chromosome is not that common. Crossing-over during meiosis occurs randomly in different positions all along the chromosome, and its occurrence frequency in the area between two genes depends on the distance between them. A short distance between genes is a very little target for crossing-over to occur, which means that only a few of them will happen, compared with the number of events between genes that are more separated between each other.  

Two genes that are very close will have a few recombination events and are strongly bounded.  

The more separated two genes are, the more chances of recombination there will be.  The closer they are, the fewer chances of recombination there will be.

Genes that express 50% of recombination frequency or more are not linked genes.  

To analyze the recombination frequency, we have to know that

1% of recombination = 1 map unit = 1centi Morgan = 1,000,000 base pairs.

And that the maximum recombination frequency is always 50%.  

The map unit is the distance between the pair of genes for which every 100 meiotic products one of them results in a recombinant one.  

In the exposed example we know that the distance of gene A from the mutation is 100 base pairs, and the distance of gene B from the mutation is 1000 base pairs.

1,000,000 base pairs ------------------ 1% recombination frequency

1000 base pairs -----------------------X = 0.001% recombination frequency

100 base pairs ------------------------ X = 0.0001% recombination frequency

According to the recombination frequency between the mutation and gene A, and between the mutation and gene B, we can assume that both genes are linked to the mutation, as they seem to be too close to it. They are so close, that their recombination frequency is very little.  

                                             

Structure of a Water molecule

Answers

Answer:

A water molecule consists of two hydrogen atoms and one oxygen atom. ... This molecular structure leads to hydrogen bonding, which is a stabilized structure in which a hydrogen atom is in a line between the oxygen atom on its own molecule and the oxygen on another molecule.

Explanation:

Which are the two most important factors determining the movement of ions across the cell membrane?

Answers

Answer:

The two most important factors determining the movement of ions across the cell membrane are: the permeability of the molecule in the membrane, and the existence of an energy source.

Explanation:

Ions are charged molecules that, no matter their size, cannot pass through the membrane by simple diffusion because of the nature of the lipid bilayer of the membrane.

Ions can move across the cell membrane in two different ways: passive transport and active transport. The disparity between these two relies on the usage of ATP (energy) - passive transport doesn't need ATP while active transport does. This is where the availability of a source of energy is important, because if there isn't, then active transport is impossible.

On the other hand, ions can move through the cell membrane without the use of energy by diffusing through the membrane with the help of specific membrane proteins that form channels for ions to pass through - and this is where permeability matters: if the cell membrane is permeable to a specific ion, it means that it has opened channels for that ion to use; and this ion will move from one fluid to the other (intracellular or extracellular) following its concentration gradient (for example, sodium is poorly concentrated in the inside of the cell, while is highly concentrated on the outside - this means that sodium will go through the membrane to get inside the cell and even the concentrations between the two fluids, but only if the membrane is permeable to sodium!).

Which is a type of star system?

dim stars
galaxies
wobbling stars
globular clusters

Answers

The type of star system is galaxies.

What is star system?

Star system is a part of solar system that comprises of different types and shapes of stars each performing it function which orbit around each other and bound by gravitational force.

Therefore, The type of star system is galaxies

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Which mechanism best describes the process by which nutrients are taken up at the apical surface of the epithelial cells that line the guy and released from their basal and lateral surfaces?

a. Proteins are tethered to the cell cortex
b. Proteins are tethered to the extracellular matrix
c. Protista are tethered to the proteins on the surface of another cell
d. Protein movement is limited by the presence of a diffusion barrier

Answers

Answer:

The correct answer is option d. "Protein movement is limited by the presence of a diffusion barrier".

Explanation:

There are transportation mechanisms that allow to take up nutrients to the apical surface of the epithelial cells. The transportation process is best describes as protein movement limited by the presence of a diffusion barrier. There are structures known as tight junctions, located just below the apical surface . These tight junctions acts as diffusion barriers, separating the  extracellular fluids surrounding the apical and basolateral membranes.

How does the formation of NAD+ differ between aerobic and anaerobic respiration?

A. NAD+ is formed in aerobic respiration by a fermentation process and formed in anaerobic respiration by oxidation of NADH.

B. NAD+ is formed by a fermentation process in anaerobic respiration by the conversion of pyruvate into lactate and by simple oxidation of NADH in aerobic respiration.

C. Under aerobic conditions, the electron acceptor is a molecule other than oxygen for NAD+ production, whereas under anaerobic conditions the electron acceptor is oxygen.

D. NAD+ is formed by the breakdown of pyruvate to form oxaloacetate in anaerobic respiration whereas in aerobic respiration it is formed by the breakdown of pyruvate into lactic acid or alcohol.

Answers

Answer:

B. NAD+ is formed by a fermentation process in anaerobic respiration by the conversion of pyruvate into lactate and by simple oxidation of NADH in aerobic respiration.

Explanation:

Nicotinamide adenine dinucleotide (NAD) is an important coenzyme that may exist in two different forms: oxidized (NAD+) and reduced (NADH). The fermentation is an anaerobic oxidation-reduction reaction where pyruvate and NADH are reactants, while lactate and NAD+ are products generated by the oxidation of NADH to NAD+ and pyruvate to lactate, respectively. On the other hand, during aerobic respiration, NADH generated by glycolysis is oxidized to form NAD+ by the donation of its electrons to reduce pyruvate to lactate. Subsequently, NAD+ is reused in glycolysis (again) in order to generate more molecules of ATP.

Temperature versus Photosynthesis Temperature in Celsius degrees Photosynthetic Rate 0 20 10 40 20 100 25 150 30 200 35 125 40 0 What is the independent variable based on the data shown? A) temperature B) plant growth C) rate of photosynthesis D) cannot determine based on information given

Answers

Answer:

A) Temperature

Explanation:

Independent variable in an experiment is the variable that the experimenter changes or manipulates in order to bring about a measurable response.

In this question given, the data is portraying the Temperature versus Photosynthetic rate. This means that the experimenter is testing to see the effect of varying temperatures on the photosynthetic rate. Hence, the TEMPERATURE is the independent variable because it is the variable being changed.


Which best describes the relationship between genetic variation and adaptation?

Answers

Answer:

Genetics are something that everyone (or only you) have. And adaptation is getting something from someone. Or Adapting to that.

Explanation:

Plz help I’ll make you brainliest if correct

Answers

Answer:

it would be C, since an abiotic factor is a non-living thing that helps shape the ecosystem.

Explanation:

If you look at A it lists flowers, which are alive. If you look at B it lists bacteria, which is alive. C doesn't list anything thats alive. D lists insects, which are alive.

Answer:

c is the ans

Explanation:

Abiotic factors include water, sunlight, oxygen, soil and temperature.

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What happened after the change in the 1950s when prison issues were termed "corrections" and ensured the basic rights of prisoners?A. Any infraction by a law enforcement official meant immediate termination.B. Judges became involved in ways to reform overcrowded prisons.C. The 1950s and 1960s became the decades of prison riots.D. Prison guards became much more humane and professional. 54. y = 1D111IB Why is it important the order we write the vertices of a triangle in a congruent statement? SELECT ALL THAT APPLY! The middle letter is the vertex of the angle. The order tells us what sides are congruent. The order does not matter The order tells us what angles are congruent. what can you infer about an ion that has a negative charge? I NEED HELP FAST FOR MY TEST!!! Do przygotowania jednej porcji gofrow potrzeba 1 i pul szklanki maki pszennej, 1i jedna trzecia szkalanki mleka oraz 0,5 szkalnki oleju pszennego. Szklanka maki wazy 16 dag, szklanka mleka-24 dag, a szklanka oleju-21,5 dag.Ile dekagramow waza lacznie skladniki potrzeba na jedna porcje gofrow? Which expression is equivalent to 1/6 (2+3)? witch are features of myth? multiply the complex number by this complex conjugate.16. 8+i17. 3 - 2i18. -7- 5i Which one is correct tho? What does it take to make big societal changes come about? Story: Mother and daughterBy: Gary SotoHow does Yollie's eighth grade fall dance impact the plot? All of the followingstatementsdescribecommandeconomiesEXCEPT for whichone? greek gods and goddess Tayler tried to evaluate the expression (22-5\times3) + 6(2253)+6left parenthesis, 22, minus, 5, times, 3, right parenthesis, plus, 6. Her work is below. \begin{aligned} &\phantom{=}(22-5\times 3) + 6\\\\ \text{Step }1:\quad&=(22 -15) + 6 \\\\ \text{Step }2:\quad&=7 + 6 \\\\ \text{Step }3:\quad&=1 \end{aligned} Step 1: Step 2: Step 3: =(2253)+6 =(2215)+6 =7+6 =1 If you own a manufacturing business that produces pants, which of the following items is considered as a supply? *zipperthreadfabricsewing machine oil The Monroe Doctrine affected, OAfrica O Asia OEurope OThe Middle East? The "goal of the United States will become to stretch the country from ocean to ocean.What two countries are preventing this from happening by controlling land on thePacific Coast? (1 point) What is the midpoint of coordinates (-3,5 and 2,-7) Answer The Following Question: Is The Following Sentance True Or False: Common Sense was published in Philadelphia in January 1776. Within months, 120,000 copies circulated throughout the colonies. Write a 1,000-word essay on the history of voting rights in America. Why did some groups receive the right to vote while others did not? Give arguments presented both in favor of and opposed to the following:14th Amendment15th Amendment19th AmendmentVoting Rights Act of 1965(I DONT WANT YOU TO WRITE WHOLE ESSAY I JUST WANT SOME HELP WITH NOTES PLEASE AND THANKYOU ALSO 100 POINTS)